| 1Heat — | Matthew Josa | SwimMAC Carolina | 0.65 | 51.61 |
| 2Heat — | Tim Phillips | SwimMAC Carolina/OSU | 0.65 | 51.68 |
| 3Heat — | Seth Stubblefield | California Aquatics | 0.62 | 51.70 |
| 4Heat — | Tom Shields | California Aquatics | 0.76 | 51.72 |
| 5Heat — | Jack Conger | Nation's Capital Swim Club/UT | 0.66 | 51.80 |
| 6Heat — | Michael Phelps | North Baltimore Aquatic Club | 0.67 | 51.87 |
| 7Heat — | Caeleb Dressel | Bolles School Sharks/FLOR | 0.61 | 52.22 |
| 8Heat — | Andrew Liang | Stanford Swimming/PASA | 0.58 | 52.65 |
| 9Heat — | Ryan Lochte | SwimMAC Carolina | 0.66 | 52.66 |
| 10Heat — | David Nolan | North Baltimore Aquatic Club/STAN | 0.67 | 52.70 |
| 11Heat — | Will Glass | City of Mobile Swim Associatio/UT | 0.69 | 52.74 |
| 12Heat — | Giles Smith | Phoenix Swim Club | 0.58 | 52.83 |
| 13Heat — | Carl Weigley | Clovis Swim Club | 0.66 | 53.24 |
| 14Heat — | Michael Andrew | Indie Swimming | 0.70 | 53.25 |
| 15Heat — | Kyler VanSwol | United States | 0.78 | 53.26 |
| 16Heat — | Andrew Sansoucie | U. of Missouri/RPLX | 0.64 | 53.28 |
| 17Heat — | Aaron Whitaker | Univ. Of Michigan Swim Team/DUNE | 0.67 | 53.31 |
| 18Heat — | Michael Miller | Duke University/DADS | 0.69 | 53.32 |
| 19Heat — | Justin Lynch | California Aquatics | 0.61 | 53.33 |
| 20Heat — | Bryce Bohman | Club Mountaineer Aquatics | 0.70 | 53.37 |
| 21Heat — | Steve Yemm | Pleasant Prairie Patriots Swim | 0.65 | 53.42 |
| 22Heat — | Nathan Lile | Gardner-Webb Univ Aquatics | 0.70 | 53.51 |
| 23Heat — | Eugene Godsoe | Wolfpack Elite | 0.62 | 53.52 |
| 24Heat — | Tripp Cooper | The Woodlands Swim Team/TENN | 0.67 | 53.54 |
| 25Heat — | Zach Harting | University of Louisville/HSA | 0.67 | 53.58 |
| 26Heat — | Josh Quallen | University of Louisville/DR | 0.69 | 53.61 |
| 26Heat — | Renny Richmond | Tucson Ford Dealers Aquatics | 0.69 | 53.61 |
| 28Heat — | Chatham Dobbs | Tucson Ford Dealers Aquatics/ARIZ | 0.67 | 53.63 |
| 29Heat — | David Jakl | Trojan Swim Club | 0.57 | 53.65 |
| 30Heat — | Matt Ellis | Longhorn Aquatics/UT | 0.68 | 53.68 |
| 31Heat — | John Shebat | Nation's Capital Swim Club/UT | 0.73 | 53.70 |
| 32Heat — | Noah Lense | Tampa Bay Aquatics | 0.64 | 53.72 |
| 33Heat — | Taylor Dale | Athens Bulldog Swim Club/UGA | 0.60 | 53.76 |
| 34Heat — | Pace Clark | Athens Bulldog Swim Club/UGA | 0.60 | 53.84 |
| 35Heat — | Tyler Mills | Tennessee Aquatics/BSC | 0.66 | 53.85 |
| 35Heat — | Matthew Grauslys | Irish Aquatics/ND | 0.72 | 53.85 |
| 37Heat — | Connor Black | Unattached | 0.69 | 53.86 |
| 37Heat — | Cody Bybee | Dayton Raiders | 0.68 | 53.86 |
| 39Heat — | Kevin Mendoza | Gaucho Aquatics/IVDA | 0.74 | 53.92 |
| 40Heat — | Vinny Tafuto | Unattached MICH | 0.65 | 53.93 |
| 41Heat — | Nick Thorne | Tucson Ford Dealers Aquatics/MHA | 0.75 | 54.03 |
| 42Heat — | Steven Tan | Harvard University | 0.65 | 54.06 |
| 43Heat — | Stewart Harrison | Buenaventura Swim Club/UOP | 0.69 | 54.07 |
| 44Heat — | John Montesi | Chelsea Piers Aquatic Club | 0.61 | 54.12 |
| 45Heat — | Alex Valente | Buenaventura Swim Club/USC | 0.61 | 54.13 |
| 46Heat — | Andrew Porter | Unattached FORK | 0.63 | 54.14 |
| 47Heat — | Logan Rysemus | Louisiana State University | 0.63 | 54.15 |
| 48Heat — | Christian McCurdy | NC State University | 0.65 | 54.19 |
| 48Heat — | Matt McHugh | Ohio State University | 0.61 | 54.19 |
| 50Heat — | Yoessef Tibazi | Gaucho Aquatics/LASC | 0.69 | 54.21 |
| 51Heat — | Michael Salazar | Ohio State University/HSA | 0.67 | 54.26 |
| 52Heat — | Nick Petersen | Schroeder YMCA Swim Team/NU | 0.65 | 54.30 |
| 53Heat — | Camden Murphy | Kingfish Aquatic Club of Water | 0.61 | 54.40 |
| 54Heat — | Bryce Keblish | Cavalier Swimming/WRAT | 0.67 | 54.42 |
| 55Heat — | Blake Atmore | SwimAtlanta/UGA | 0.62 | 54.47 |
| 56Heat — | Wesley Coles | Unattached FPU | 0.67 | 54.48 |
| 57Heat — | Ben Schafer | Princeton University Swim Team | 0.63 | 54.51 |
| 58Heat — | Greg Brocato | Central Bucks Swim Team | 0.71 | 54.53 |
| 58Heat — | Mark Sarman | Unattached/NCAP | 0.67 | 54.53 |
| 60Heat — | David Dingess | Kentucky Aquatics/LINS | 0.70 | 54.55 |
| 61Heat — | William Pisani | Club Seminole | 0.72 | 54.56 |
| 62Heat — | Aaron Young | Canby Swim Team | 0.60 | 54.58 |
| 63Heat — | Kyle Higgins | Kentucky Aquatics | 0.65 | 54.60 |
| 63Heat — | Mike Thomas | California Aquatics/UDAC | 0.66 | 54.60 |
| 65Heat — | Bobby Bollier | Stanford Swimming | 0.63 | 54.63 |
| 66Heat — | Aaron Schultz | NOVA of Virginia Aquatics, Inc | 0.73 | 54.66 |
| 67Heat — | Jake Johnson | Delaware Swim Team | 0.66 | 54.70 |
| 68Heat — | Liam McCloskey | Rancho San Dieguito/AU | 0.66 | 54.72 |
| 68Heat — | Michael Jensen | Upper Dublin Aquatic Club | 0.70 | 54.72 |
| 70Heat — | James Bretscher | Tennessee Aquatics/NCS | 0.62 | 54.75 |
| 71Heat — | Jimmy Yoder | Stingrays | 0.66 | 54.76 |
| 72Heat — | Harrison Tran | Wisconsin Aquatics/DM | 0.67 | 54.78 |
| 73Heat — | Andrew Marsh | Club Mountaineer Aquatics/WVU | 0.62 | 54.81 |
| 74Heat — | Jacob Kelsoe | University Of Alabama Swim Clu | 0.64 | 54.83 |
| 75Heat — | Sam Wilcher | Alligator Aquatics | 0.74 | 54.84 |
| 75Heat — | Benjamin Southern | Yellow Jacket/NCAP | 0.66 | 54.84 |
| 77Heat — | David Boland | University of Louisville/CW | 0.64 | 54.87 |
| 78Heat — | Sam Pomajevich | Nation's Capital Swim Club | 0.66 | 54.90 |
| 78Heat — | Matt Conway | North Carolina Aquatic Club | 0.67 | 54.90 |
| 80Heat — | Max Yakubovich | Harvard University | 0.60 | 54.97 |
| 81Heat — | Nicolas Albiero | Cardinal Aquatics | 0.67 | 54.99 |
| 81Heat — | Franco Reyes | Unattached WEST/WEST | 0.58 | 54.99 |
| 83Heat — | Lucas Bureau | H2okie Aquatics/VTECH | 0.66 | 55.01 |
| 83Heat — | Ryan Hoffer | Scottsdale Aquatic Club | 0.67 | 55.01 |
| 85Heat — | Martin Wallace | U. of Missouri/JH | 0.70 | 55.04 |
| 85Heat — | Patrick Park | Pitchfork Aquatics/ASU | 0.65 | 55.04 |
| 87Heat — | Ben Kennedy | Carter Center Aquatics/FLOR | 0.65 | 55.05 |
| 88Heat — | Micah Ornelas | Unattached | 0.64 | 55.06 |
| 89Heat — | Gregory Nymberg | Ohio State University/CAC | 0.66 | 55.09 |
| 90Heat — | Ryan McCoy | Phoenix Swim Club/SLU | 0.65 | 55.10 |
| 91Heat — | Cameron Craig | Unattached CW | 0.67 | 55.11 |
| 92Heat — | Nicholas Loomis | Unattached | 0.66 | 55.14 |
| 93Heat — | Kyle Whitaker | Club Wolverine | 0.65 | 55.16 |
| 94Heat — | Max Holter | Fort Collins Area Swim Team/UT | 0.77 | 55.17 |
| 94Heat — | Kyle Grissom | Trojan Swim Club/TNT | 0.65 | 55.17 |
| 94Heat — | Teo D'Alessandro | Princeton University Swim Team/BSS | 0.68 | 55.17 |
| 94Heat — | Luke Reisch | Fairport Area Swim Team | 0.66 | 55.17 |
| 98Heat — | Micah Slaton | Nitro Swimming | 0.68 | 55.24 |
| 98Heat — | Austin Flager | Boilermaker Aquatics/NASA | 0.68 | 55.24 |
| 100Heat — | Kyle Ward | Splash Club Inc | 0.67 | 55.25 |
| 101Heat — | Michael Flach | Club Wolverine | 0.60 | 55.28 |
| 102Heat — | John Martens | Longhorn Aquatics/UT | 0.68 | 55.32 |
| 103Heat — | Ryan Huizing | Northwest Aquatic Club Swimmin | 0.66 | 55.33 |
| 104Heat — | Andrey Tretyakov | Madison Swimming Association | 0.61 | 55.34 |
| 104Heat — | Bryce Mefford | Sierra Marlins Swim Team | 0.64 | 55.34 |
| 104Heat — | Aaron Gustafson | Longhorn Aquatics/UT | 0.62 | 55.34 |
| 107Heat — | Tyler Rauth | Southern Methodist University | 0.65 | 55.35 |
| 108Heat — | Ariel Spektor | Bolles School Sharks | 0.66 | 55.36 |
| 109Heat — | Zachary Smith | Crimson Tide Aquatics | 0.69 | 55.39 |
| 110Heat — | Luke Mankus | U. of Missouri/MTRO | 0.64 | 55.42 |
| 111Heat — | Wade Allen | Gaucho Aquatics | 0.65 | 55.43 |
| 112Heat — | Dylan Tarazona | Team Rebel Aquatics/TRA | 0.70 | 55.44 |
| 113Heat — | Foster Ballard | Auburn University Swim Club | 0.65 | 55.50 |
| 114Heat — | Spencer Carl | Unattached MLA | 0.68 | 55.51 |
| 115Heat — | Mike Dugan | Buffalo Area Aquatic Club | 0.74 | 55.53 |
| 115Heat — | Daryl Turner | University of Minnesota/ACES | 0.71 | 55.53 |
| 117Heat — | Noah Henry | Tigershark Swim Team | 0.71 | 55.54 |
| 117Heat — | Ryan Dunne | Nation's Capital Swim Club/AFA | 0.73 | 55.54 |
| 119Heat — | Matthew Yish | SOLO Aquatics | 0.68 | 55.56 |
| 120Heat — | Michael Arnold | Chattahoochee Gold Swim Club | 0.66 | 55.60 |
| 120Heat — | Thomas Glenn | Jersey Wahoos/Brown | 0.77 | 55.60 |
| 122Heat — | Kyle Patnode | University Of Iowa | 0.68 | 55.61 |
| 123Heat — | Ryan Tate | Northwestern University ST/SO | 0.64 | 55.63 |
| 124Heat — | Mathias Oh | King Aquatic Club/UofA | 0.68 | 55.65 |
| 124Heat — | Drew Modrov | Swimmin | 0.73 | 55.65 |
| 126Heat — | Will Macmillan | North Carolina Aquatic Club | 0.71 | 55.74 |
| 127Heat — | Jacob Huerta | Coastal Bend Allstars | 0.71 | 55.78 |
| 128Heat — | Thaxter Hensley | Blue Dolfins/FSU | 0.71 | 55.99 |
| 129Heat — | Zachary Poti | Blue Dolfins | 0.75 | 56.06 |
| 130Heat — | Grant Shoults | Mission Viejo Nadadores | 0.64 | 56.08 |
| 131Heat — | Will Freeman | University Of Alabama Swim Clu | 0.64 | 56.75 |
| 132Heat — | Mark Jurek | Arizona Gold Swimming | 0.72 | 56.79 |
| 133Heat — | Billy Beard | Upper Main Line YMCA | 0.65 | 57.29 |
| —Heat — | Mick Litherland | Dynamo Swim Club/UGA | 0.69 | DQ |
| —Heat — | David Williams | Wolfpack Elite | — | |
| —Heat — | Andrew Seliskar | California Aquatics/NCAP | — | |
| —Heat — | Ryan Held | NC State University | — | |
| —Heat — | Joe Bonk | YMCA of the Triangle Area Swim/NCS | — | NS |