| 1Heat — | Jacob Pebley | California Aquatics/CAT | 0.64 | 1:56.29 |
| 2Heat — | Tyler Clary | SwimMAC Carolina/MICH | 0.53 | 1:56.85 |
| 3Heat — | Ryan Murphy | California Aquatics | 0.58 | 1:57.35 |
| 4Heat — | Michael Taylor | Dynamo Swim Club | 0.59 | 1:57.92 |
| 5Heat — | Robert Owen | H2okie Aquatics/CRIM | 0.55 | 1:58.01 |
| 6Heat — | Sean Lehane | University Of Tennessee | 0.62 | 1:58.04 |
| 7Heat — | Hennessey Stuart | Unattached NCS/NCS | 0.55 | 1:58.32 |
| 8Heat — | Austin Katz | Sarasota YMCA Sharks | 0.56 | 1:59.05 |
| 9Heat — | Jay Litherland | Dynamo Swim Club/UGA | 0.72 | 1:59.30 |
| 10Heat — | Joey Reilman | Tennessee Aquatics/FAST | 0.64 | 1:59.55 |
| 11Heat — | Justin Ress | Marlins of Raleigh/NCSU | 0.69 | 1:59.60 |
| 12Heat — | Carter Griffin | DU Hilltoppers/UMIZ | 0.67 | 1:59.74 |
| 13Heat — | Patrick Mulcare | Trojan Swim Club/USC | 0.71 | 1:59.86 |
| 14Heat — | Matt Grevers | Tucson Ford Dealers Aquatics | 0.67 | 2:00.03 |
| 15Heat — | Drew Cosgarea | North Baltimore Aquatic Club/STAN | 0.66 | 2:00.04 |
| 16Heat — | Jake Taylor | Brigham Young University Swim/CVM | 0.59 | 2:00.31 |
| 17Heat — | Tristan Sanders | Univ. Of Michigan Swim Team/PYP | 0.59 | 2:00.88 |
| 18Heat — | Cole Bateman | Eastern Michigan University | 0.64 | 2:01.10 |
| 19Heat — | Patrick Conaton | Badger Swim Club Inc/STAN | 0.66 | 2:01.20 |
| 20Heat — | Bryce Mefford | Sierra Marlins Swim Team | 0.66 | 2:01.21 |
| 21Heat — | Aaron Greene | University of Louisville/NTN | 0.70 | 2:01.22 |
| 22Heat — | James Beckman | Indiana University/MUNS | 0.59 | 2:01.41 |
| 23Heat — | Alessandro Boratto | Upper Dublin Aquatic Club | 0.62 | 2:01.79 |
| 24Heat — | Mike Thomas | California Aquatics/UDAC | 0.61 | 2:01.81 |
| 24Heat — | Rob Whitacre | Irish Aquatics/ND | 0.61 | 2:01.81 |
| 26Heat — | Taylor Delk | SwimAtlanta | 0.60 | 2:01.84 |
| 27Heat — | Sean Workman | H2okie Aquatics/EDWY | 0.51 | 2:01.91 |
| 28Heat — | Chris Devlin | Upper Dublin Aquatic Club | 0.64 | 2:02.07 |
| 29Heat — | Nolan Tesone | University of Louisville/Jeffco | 0.59 | 2:02.08 |
| 30Heat — | Brennan Balogh | Lincoln Select Swimming/FLOR | 0.51 | 2:02.15 |
| 31Heat — | Grant Proctor | University of Pennsylvania/JW | 0.64 | 2:02.17 |
| 32Heat — | Corey Okubo | Princeton U/AZOT | 0.60 | 2:02.19 |
| 33Heat — | Garrett Powell | Athens Bulldog Swim Club/UGA | 0.62 | 2:02.46 |
| 34Heat — | Thane Maudslien | Tucson Ford Dealers Aquatics/KING | 0.58 | 2:02.51 |
| 35Heat — | Bob Glover | Indiana University/GOAL | 0.57 | 2:02.54 |
| 35Heat — | Matthew Klotz | Louisiana State University | 0.59 | 2:02.54 |
| 37Heat — | Josh Artmann | Austin Swim Club | 0.61 | 2:02.62 |
| 38Heat — | Taylor Dale | Athens Bulldog Swim Club/UGA | 0.65 | 2:02.74 |
| 39Heat — | Jonathan Lieberman | Northwestern University ST | 0.64 | 2:02.77 |
| 40Heat — | Griffin Schaetzle | U. of Missouri/EAC | 0.61 | 2:02.78 |
| 41Heat — | Luke Papendick | Univ. Of Michigan Swim Team/LHY | 0.67 | 2:02.86 |
| 42Heat — | Nicolas Albiero | Cardinal Aquatics | 0.61 | 2:02.91 |
| 43Heat — | Todd McCarthy | Wisconsin Aquatics | 0.76 | 2:02.95 |
| 44Heat — | Preston Jenkins | Brigham Young University Swim/BSS | 0.54 | 2:02.98 |
| 45Heat — | Crews Wellford | University Of Alabama Swim Clu | 0.65 | 2:03.01 |
| 46Heat — | Jacob Siar | Wolfpack Elite/NCS | 0.59 | 2:03.02 |
| 47Heat — | Ryan Ball | Wyoming Aquatics/WYO | 0.56 | 2:03.14 |
| 48Heat — | Austin Byrd | Unattached | 0.57 | 2:03.18 |
| 49Heat — | Brandon James | Walnut Creek Aquabears | 0.67 | 2:03.26 |
| 50Heat — | Will Stange | Princeton Piranh/Cornell | 0.59 | 2:03.33 |
| 51Heat — | Jack Mangan | Univ. Of Michigan Swim Team | 0.60 | 2:03.35 |
| 52Heat — | Chris Thames | Metro Atlanta Aquatic Club | 0.60 | 2:03.36 |
| 53Heat — | Clark Beach | Quest Swimming | 0.65 | 2:03.45 |
| 54Heat — | Daniel Carr | Pikes Peak Athletics | 0.66 | 2:03.50 |
| 55Heat — | Griffin Alaniz | Rockville Montgomery Swim Club | 0.62 | 2:03.60 |
| 56Heat — | Heyward Harrison | Lakeside Swim Team/UNC | 0.60 | 2:03.63 |
| 57Heat — | Austin Vacek | Katy Aquatic Team For Youth | 0.76 | 2:03.71 |
| 58Heat — | Rob Zofchak | Club Wolverine | 0.69 | 2:03.88 |
| 58Heat — | Matthew O'Donnell | NC State University | 0.67 | 2:03.88 |
| 60Heat — | John Healy | Unattached MOR | 0.63 | 2:03.90 |
| 61Heat — | Nathan Murray | SwimMAC Carolina | 0.68 | 2:04.00 |
| 62Heat — | Zachary Lierley | University of Pittsburgh | 0.69 | 2:04.01 |
| 63Heat — | Braxton Moore | Cypress Fairbanks Swim Club | 0.60 | 2:04.04 |
| 64Heat — | Zachary Poti | Blue Dolfins | 0.61 | 2:04.08 |
| 65Heat — | Brandon Goldman | Louisiana State University | 0.68 | 2:04.13 |
| 66Heat — | Nick Alexander | Flyers Aquatic Swim Team | 0.69 | 2:04.27 |
| 67Heat — | Curtis Ogren | Stanford Swimming/PASA | 0.60 | 2:04.28 |
| 68Heat — | Daniel Tran | Harvard University/AZOT | 0.56 | 2:04.30 |
| 69Heat — | Sam Cuthbert | NOVA of Virginia Aquatics, Inc | 0.62 | 2:04.33 |
| 70Heat — | Jared Smith | University of Denver Hilltoppe/DU | 0.57 | 2:04.38 |
| 71Heat — | Dean Farris | Metro Atlanta Aquatic Club | 0.64 | 2:04.40 |
| 72Heat — | Cary Wright | Trojan Swim Club | 0.61 | 2:04.45 |
| 72Heat — | Cole Buese | Princeton U/NBAC | 0.59 | 2:04.45 |
| 72Heat — | Grant Kelton | U. of Missouri/MTAC | 0.62 | 2:04.45 |
| 75Heat — | Anthony Kim | Charger Aquatics | 0.68 | 2:04.48 |
| 76Heat — | Connor Kalisz | North Baltimore Aquatic Club/FSU | 0.75 | 2:04.51 |
| 77Heat — | Jordan Ross | SwimAtlanta/MICH | 0.70 | 2:04.54 |
| 78Heat — | Coleman Stewart | North Baltimore Aquatic Club | 0.62 | 2:04.74 |
| 79Heat — | Matt Lujan | Tucson Ford Dealers Aquatics/ARIZ | 0.61 | 2:04.85 |
| 80Heat — | Colton Williamson | Yellow Jacket/DYNA | 0.70 | 2:05.04 |
| 81Heat — | Wade O'Brien | NOVA of Virginia Aquatics, Inc | 0.61 | 2:05.05 |
| 82Heat — | James Wells | Badger Swim Club Inc/IND | 0.64 | 2:05.19 |
| 83Heat — | Tyler Lis | Northwestern University ST/RACE | 0.61 | 2:05.21 |
| 84Heat — | Ian Lemaistre | Longhorn Aquatics/UT | 0.71 | 2:05.22 |
| 85Heat — | Sam Stewart | Crawfish Aquatics | 0.69 | 2:05.29 |
| 86Heat — | Brandon Shreeve | Corvallis Aquatic Team/UTAH | 0.62 | 2:05.31 |
| 87Heat — | Brendan Feehery | Lakeside Aquatic Club | 0.68 | 2:05.45 |
| 88Heat — | Nick Carter | Gulliver Swim Club | 0.60 | 2:05.51 |
| 89Heat — | Parker Campbell | Wolfpack Elite/NCS | 0.59 | 2:05.55 |
| 90Heat — | Matthew Garcia | Sarasota YMCA Sharks | 0.59 | 2:05.61 |
| 91Heat — | Barkley Perry | Pitchfork Aquatics/ASU | 0.63 | 2:05.62 |
| 92Heat — | Dylan Davis | North Carolina Aquatic Club | 0.62 | 2:05.65 |
| 93Heat — | Harrison Haines | Gator Swim Club | 0.63 | 2:05.74 |
| 94Heat — | Kevin Liu | Unattached USCS/USCS | 0.60 | 2:05.83 |
| 95Heat — | Joe Young | Boilermaker Aquatics/SSC | 0.57 | 2:05.90 |
| 96Heat — | Curtis Klein | Lake Oswego Swim Club/TIGR | 0.77 | 2:05.92 |
| 97Heat — | Matthew Marquardt | Mercy Healthplex Sea Wolves | 0.61 | 2:06.35 |
| 98Heat — | Justin Nguyen | Irvine Novaquatics | 0.63 | 2:06.45 |
| 99Heat — | Ethan Beach | Quest Swimming | 0.66 | 2:06.65 |
| 100Heat — | Ted Schubert | NOVA of Virginia Aquatics, Inc | 0.66 | 2:06.74 |
| 101Heat — | Benjamin Ho | Palo Alto Stanford Aquatics | 0.67 | 2:07.09 |
| 102Heat — | Thomas Smith | Louisiana State University | 0.58 | 2:07.32 |
| 103Heat — | Tal Davis | Team Greenville | 0.68 | 2:07.53 |
| 104Heat — | Kevin Stang | Yale University/LESD | 0.67 | 2:07.72 |
| 105Heat — | Jesse Ryckman | California Aquatics/CLASS | 0.76 | 2:08.17 |
| 106Heat — | Peter Larson | Edina Swim Club | 0.67 | 2:08.18 |
| 107Heat — | Ethan Young | Carpet Capital Aquatics Club | 0.67 | 2:08.84 |
| 108Heat — | Ryan Gordon | University at Buffalo Swim Tea | 0.58 | 2:08.85 |
| 109Heat — | Jason Park | Metroplex Aquatics | 0.65 | 2:08.94 |
| —Heat — | Augustus Whiteman | Nitro Swimming/OSU | 0.72 | DQ |
| —Heat — | Jonathan Roberts | North Texas Nadadores/UT | — | |
| —Heat — | David Crossland | Delaware Swim Team | — | |
| —Heat — | Sean Grieshop | Nitro Swimming | — | |
| —Heat — | Will Raynor | Univ. Of Michigan Swim Team | — | NS |