| 1Heat — | David Plummer | New York Athletic Club | 0.61 | 53.22 |
| 2Heat — | Jacob Pebley | California Aquatics/CAT | 0.64 | 53.48 |
| 3Heat — | Michael Taylor | Dynamo Swim Club | 0.61 | 53.77 |
| 4Heat — | Ryan Murphy | California Aquatics | 0.59 | 53.84 |
| 5Heat — | Matt Grevers | Tucson Ford Dealers Aquatics | 0.65 | 53.98 |
| 6Heat — | Sean Lehane | University Of Tennessee | 0.55 | 54.49 |
| 7Heat — | Jake Taylor | Brigham Young University Swim/CVM | 0.54 | 54.50 |
| 8Heat — | Eugene Godsoe | Wolfpack Elite | 0.55 | 54.74 |
| 9Heat — | John Shebat | Nation's Capital Swim Club/UT | 0.66 | 54.91 |
| 10Heat — | Patrick Mulcare | Trojan Swim Club/USC | 0.65 | 54.94 |
| 11Heat — | Taylor Dale | Athens Bulldog Swim Club/UGA | 0.62 | 54.95 |
| 12Heat — | Justin Ress | Marlins of Raleigh/NCSU | 0.66 | 55.00 |
| 12Heat — | Ryan Harty | Longhorn Aquatics/UT | 0.63 | 55.00 |
| 14Heat — | Hennessey Stuart | Unattached NCS/NCS | 0.53 | 55.40 |
| 15Heat — | Carter Griffin | DU Hilltoppers/UMIZ | 0.59 | 55.41 |
| 16Heat — | Austin Katz | Sarasota YMCA Sharks | 0.57 | 55.41 |
| 17Heat — | Cameron Craig | Unattached CW | 0.55 | 55.41 |
| 18Heat — | Luke Kaliszak | University Of Alabama Swim Clu | 0.58 | 55.43 |
| 19Heat — | Bob Glover | Indiana University/GOAL | 0.54 | 55.43 |
| 20Heat — | Andrew Marsh | Club Mountaineer Aquatics/WVU | 0.63 | 55.63 |
| 21Heat — | Chatham Dobbs | Tucson Ford Dealers Aquatics/ARIZ | 0.56 | 55.67 |
| 22Heat — | Bryce Mefford | Sierra Marlins Swim Team | 0.67 | 55.70 |
| 23Heat — | Luke Papendick | Univ. Of Michigan Swim Team/LHY | 0.66 | 55.74 |
| 24Heat — | Jacob Siar | Wolfpack Elite/NCS | 0.59 | 55.78 |
| 25Heat — | Robert Owen | H2okie Aquatics/CRIM | 0.55 | 55.82 |
| 26Heat — | Mark McGlaughlin | Iowa Flyers Swim Club | 0.62 | 55.88 |
| 27Heat — | Brandon Goldman | Louisiana State University | 0.58 | 55.89 |
| 27Heat — | James Wells | Badger Swim Club Inc/IND | 0.63 | 55.89 |
| 27Heat — | Joey Reilman | Tennessee Aquatics/FAST | 0.65 | 55.89 |
| 30Heat — | Bryce Bohman | Club Mountaineer Aquatics | 0.66 | 55.91 |
| 31Heat — | Alessandro Boratto | Upper Dublin Aquatic Club | 0.64 | 55.96 |
| 32Heat — | Nicolas Albiero | Cardinal Aquatics | 0.55 | 55.98 |
| 33Heat — | Cole Bateman | Eastern Michigan University | 0.62 | 56.02 |
| 34Heat — | Drew Teduits | Madison Aquatic Club | 0.69 | 56.04 |
| 34Heat — | Kyle Darmody | Auburn University Swim Club/MAC | 0.66 | 56.04 |
| 36Heat — | Daniel Carr | Pikes Peak Athletics | 0.64 | 56.05 |
| 37Heat — | Preston Jenkins | Brigham Young University Swim/BSS | 0.56 | 56.06 |
| 38Heat — | Patrick Conaton | Badger Swim Club Inc/STAN | 0.63 | 56.08 |
| 38Heat — | Grant Betulius | Tucson Ford Dealers Aquatics | 0.65 | 56.08 |
| 40Heat — | Aaron Greene | University of Louisville/NTN | 0.65 | 56.09 |
| 41Heat — | Matthew Klotz | Louisiana State University | 0.60 | 56.10 |
| 42Heat — | David Jakl | Trojan Swim Club | 0.55 | 56.12 |
| 43Heat — | Jon Knox | Trojan Swim Club/USC | 0.70 | 56.16 |
| 44Heat — | Austin Byrd | Unattached | 0.61 | 56.19 |
| 45Heat — | Crews Wellford | University Of Alabama Swim Clu | 0.61 | 56.20 |
| 46Heat — | Will Glass | City of Mobile Swim Associatio/UT | 0.62 | 56.23 |
| 47Heat — | Grant Kelton | U. of Missouri/MTAC | 0.60 | 56.28 |
| 48Heat — | Nick Petersen | Schroeder YMCA Swim Team/NU | 0.56 | 56.29 |
| 48Heat — | Logan Rysemus | Louisiana State University | 0.60 | 56.29 |
| 50Heat — | Rob Zofchak | Club Wolverine | 0.58 | 56.30 |
| 51Heat — | Michael Baric | Wolfpack Elite | 0.60 | 56.31 |
| 52Heat — | Tristan Sanders | Univ. Of Michigan Swim Team/PYP | 0.58 | 56.35 |
| 53Heat — | Daryl Turner | University of Minnesota/ACES | 0.55 | 56.37 |
| 54Heat — | Kyle Dudzinski | Univ. Of Michigan Swim Team | 0.63 | 56.38 |
| 55Heat — | Dean Farris | Metro Atlanta Aquatic Club | 0.64 | 56.39 |
| 56Heat — | Collin Higgins | SwimMAC Carolina/VTECH | 0.54 | 56.43 |
| 57Heat — | Ethan Young | Carpet Capital Aquatics Club | 0.61 | 56.44 |
| 58Heat — | Justin McArthur | Swim Utah | 0.61 | 56.49 |
| 59Heat — | Michael Pilyugin | H2okie Aquatics/SBU | 0.53 | 56.53 |
| 60Heat — | James Beckman | Indiana University/MUNS | 0.58 | 56.55 |
| 61Heat — | Rob Whitacre | Irish Aquatics/ND | 0.60 | 56.56 |
| 61Heat — | Thomas Trace | Ohio State University/UA | 0.60 | 56.56 |
| 61Heat — | Christian Garkani | University of Louisville/AZOT | 0.66 | 56.56 |
| 64Heat — | Connor Kalisz | North Baltimore Aquatic Club/FSU | 0.73 | 56.58 |
| 64Heat — | Thomas Anderson | King Aquatic Club | 0.60 | 56.58 |
| 66Heat — | Aaron Whitaker | Univ. Of Michigan Swim Team/DUNE | 0.60 | 56.62 |
| 67Heat — | Austin Quinn | Cavalier Swimming | 0.63 | 56.63 |
| 68Heat — | Clark Beach | Quest Swimming | 0.64 | 56.66 |
| 68Heat — | Will Manion | X-Cel Swimming/W&M | 0.57 | 56.66 |
| 68Heat — | Max St George | Duke University/BRRY | 0.67 | 56.66 |
| 71Heat — | Glen Cowand | Katy Aquatic Team For Youth | 0.58 | 56.72 |
| 72Heat — | Jack Mangan | Univ. Of Michigan Swim Team | 0.61 | 56.73 |
| 72Heat — | Ian Lemaistre | Longhorn Aquatics/UT | 0.62 | 56.73 |
| 72Heat — | Mike Thomas | California Aquatics/UDAC | 0.59 | 56.73 |
| 72Heat — | Nolan Tesone | University of Louisville/Jeffco | 0.57 | 56.73 |
| 76Heat — | Josh Artmann | Austin Swim Club | 0.55 | 56.76 |
| 77Heat — | Jared Smith | University of Denver Hilltoppe/DU | 0.56 | 56.77 |
| 78Heat — | Sean Feher | Springfield Aquatics | 0.68 | 56.78 |
| 78Heat — | Brandon James | Walnut Creek Aquabears | 0.65 | 56.78 |
| 80Heat — | Brad Shannon | Ohio State University/CBAC | 0.63 | 56.81 |
| 81Heat — | John Montesi | Chelsea Piers Aquatic Club | 0.59 | 56.84 |
| 82Heat — | Chris Thames | Metro Atlanta Aquatic Club | 0.63 | 56.87 |
| 83Heat — | Joshua Swart | Kentucky Aquatics/SCAT | 0.60 | 56.88 |
| 84Heat — | Aaron Young | Canby Swim Team | 0.64 | 56.90 |
| 85Heat — | Kyle Robrock | University of Denver Hilltoppe/DU | 0.56 | 56.91 |
| 85Heat — | Brennan Balogh | Lincoln Select Swimming/FLOR | 0.57 | 56.91 |
| 87Heat — | Makoa Haneberg | Tiger Aquatics/UOP | 0.61 | 56.99 |
| 88Heat — | Adam Mania | Schroeder YMCA Swim Team | 0.60 | 57.04 |
| 88Heat — | Josh Quallen | University of Louisville/DR | 0.63 | 57.04 |
| 90Heat — | Braxton Moore | Cypress Fairbanks Swim Club | 0.60 | 57.05 |
| 90Heat — | Matt Lujan | Tucson Ford Dealers Aquatics/ARIZ | 0.58 | 57.05 |
| 92Heat — | Tim Maurer | Penn State University/HAC | 0.67 | 57.06 |
| 93Heat — | Michael Chadwick | U. of Missouri/MAC | 0.52 | 57.12 |
| 94Heat — | Sean Workman | H2okie Aquatics/EDWY | 0.49 | 57.14 |
| 94Heat — | Jonathan Boland | Gamecock Aquatics/PALM | 0.54 | 57.14 |
| 94Heat — | Will Stange | Princeton Piranh/Cornell | 0.58 | 57.14 |
| 94Heat — | Taylor Delk | SwimAtlanta | 0.59 | 57.14 |
| 98Heat — | Chris Devlin | Upper Dublin Aquatic Club | 0.60 | 57.15 |
| 98Heat — | Benjamin Lin | Summit Area YMCA/WILL | 0.66 | 57.15 |
| 100Heat — | Daniel Hein | Kishwaukee YMCA Dekalb County | 0.58 | 57.17 |
| 101Heat — | Joe Young | Boilermaker Aquatics/SSC | 0.54 | 57.19 |
| 102Heat — | Zach Switzer | H2okie Aquatics/VTECH | 0.56 | 57.20 |
| 102Heat — | Drew Kibler | Carmel Swim Club | 0.60 | 57.20 |
| 104Heat — | Harrison Haines | Gator Swim Club | 0.65 | 57.23 |
| 105Heat — | Jacob DeMercurio | Unattached TFA | 0.59 | 57.24 |
| 106Heat — | Will Countie | Marlins of Raleigh | 0.63 | 57.25 |
| 106Heat — | Jeff Natalizio | Gators Swim Club | 0.56 | 57.25 |
| 108Heat — | Taylor Copeland | Auburn University Swim Club | 0.57 | 57.26 |
| 108Heat — | Jack Dolan | Rockwood Swim Club | 0.67 | 57.26 |
| 108Heat — | Alex Walton | U. of Missouri/ICE | 0.60 | 57.26 |
| 111Heat — | Chase Bloch | Unattached CLSS | 0.61 | 57.27 |
| 112Heat — | Nathan Lile | Gardner-Webb Univ Aquatics | 0.61 | 57.29 |
| 112Heat — | Walker Thaning | Kentucky Aquatics/BSW | 0.62 | 57.29 |
| 114Heat — | Parker Campbell | Wolfpack Elite/NCS | 0.60 | 57.31 |
| 114Heat — | Augustus Whiteman | Nitro Swimming/OSU | 0.67 | 57.31 |
| 114Heat — | Jack Manchester | Harvard University | 0.62 | 57.31 |
| 117Heat — | Connor Oslin | University Of Alabama Swim Clu | 0.58 | 57.32 |
| 117Heat — | Nick Carter | Gulliver Swim Club | 0.58 | 57.32 |
| 119Heat — | Tan Dunn | Southern Crescent Aquatic Team | 0.62 | 57.34 |
| 119Heat — | Austin Vacek | Katy Aquatic Team For Youth | 0.74 | 57.34 |
| 121Heat — | Luca Spinazzola | Trojan Swim Club | 0.62 | 57.35 |
| 122Heat — | Jacob Kelsoe | University Of Alabama Swim Clu | 0.66 | 57.36 |
| 122Heat — | Patrick Riess | North Carolina Aquatic Club | 0.64 | 57.36 |
| 122Heat — | Ryan O'Donnell | McFarland Spartan Sharks | 0.64 | 57.36 |
| 125Heat — | Alex Toetz | Boilermaker Aquatics/PUR | 0.60 | 57.37 |
| 126Heat — | Matt McHugh | Ohio State University | 0.63 | 57.38 |
| 127Heat — | Shayne Fleming | De Anza Cupertino Aquatics | 0.54 | 57.41 |
| 128Heat — | Matthew Garcia | Sarasota YMCA Sharks | 0.57 | 57.47 |
| 129Heat — | Hudson Carroll | University at Bu/HAWK | 0.56 | 57.48 |
| 129Heat — | Dylan Curtis | Dayton Raiders | 0.65 | 57.48 |
| 131Heat — | Mark Belanger | Ohio State University/FAST | 0.55 | 57.49 |
| 131Heat — | Michael Cohn | UC San Diego/RSD | 0.65 | 57.49 |
| 131Heat — | Tyler Sesvold | St Charles Swim Team | 0.58 | 57.49 |
| 134Heat — | Mitchell Stover | Wright State University | 0.63 | 57.53 |
| 135Heat — | Coleman Stewart | North Baltimore Aquatic Club | 0.60 | 57.57 |
| 136Heat — | Cole Cogswell | Canyons Aquatic Club | 0.58 | 57.58 |
| 137Heat — | David Puczkowski | CLASS Aquatics/CAL | 0.67 | 57.59 |
| 138Heat — | Dominic Breschi | Towson University Swimming/LBA | 0.68 | 57.63 |
| 138Heat — | Noah Henry | Tigershark Swim Team | 0.67 | 57.63 |
| 140Heat — | J Todd Wilson | Lancaster Aquatic Club | 0.61 | 57.64 |
| 141Heat — | Jack Walsh | Stanford Swimming | 0.66 | 57.65 |
| 142Heat — | Robert Borowicz | Yellow Jacket/YSSC | 0.54 | 57.66 |
| 142Heat — | Andrew Jovanovic | Northwestern University ST/WILD | 0.58 | 57.66 |
| 144Heat — | Thomas Smith | Louisiana State University | 0.55 | 57.77 |
| 144Heat — | Jacob Molacek | Greater Omaha Aquatics | 0.66 | 57.77 |
| 146Heat — | Nicholas Salyers | SwimAtlanta/UGA | 0.62 | 57.82 |
| 147Heat — | Ethan King | Navy Swimming | 0.65 | 57.83 |
| 148Heat — | Jake Lamparella | Eagle Swim Team Inc | 0.60 | 57.85 |
| 149Heat — | Jase Ashkin | Rockville Montgomery Swim Club | 0.62 | 57.94 |
| 150Heat — | Alexander Lewis | Princeton U/GYWD | 0.53 | 57.95 |
| 151Heat — | Wesley McGovern | Tampa Bay Aquatics/PUR | 0.69 | 57.97 |
| 152Heat — | Kyle Maas | Unattached MLA | 0.64 | 58.02 |
| 152Heat — | Nick Alexander | Flyers Aquatic Swim Team | 0.67 | 58.02 |
| 152Heat — | Jimmy Dagley | University Of Tennessee/HEAT | 0.52 | 58.02 |
| 155Heat — | Anthony Kim | Charger Aquatics | 0.62 | 58.04 |
| 155Heat — | Kevin Liu | Unattached USCS/USCS | 0.58 | 58.04 |
| 157Heat — | Brad Oberg | Yellow Jacket/YSSC | 0.60 | 58.07 |
| 157Heat — | Benjamin Ho | Palo Alto Stanford Aquatics | 0.64 | 58.07 |
| 157Heat — | David Carlson | Navy Swimming | 0.68 | 58.07 |
| 157Heat — | Luke Burton | SwimMAC Carolina/DAV | 0.70 | 58.07 |
| 161Heat — | Brandon Presswood | Tiger Aquatics/UOP | 0.63 | 58.08 |
| 162Heat — | Ryan Hoffer | Scottsdale Aquatic Club | 0.64 | 58.10 |
| 162Heat — | Aaron Gustafson | Longhorn Aquatics/UT | 0.73 | 58.10 |
| 162Heat — | Connor Bos | Gardner-Webb Univ Aquatics | 0.55 | 58.10 |
| 165Heat — | Khalil Fonder | Virginia Gators | 0.56 | 58.13 |
| 166Heat — | Dylan Davis | North Carolina Aquatic Club | 0.62 | 58.16 |
| 167Heat — | Zachary Poti | Blue Dolfins | 0.58 | 58.27 |
| 168Heat — | Jacob Wallace | Unattached AGS | 0.62 | 58.29 |
| 169Heat — | Henrik Pohlmann | Cavalier Swimming | 0.69 | 58.33 |
| 169Heat — | Alex Robinson | Memphis Tiger Swim Club | 0.48 | 58.33 |
| 171Heat — | Jesse Ryckman | California Aquatics/CLASS | 0.75 | 58.38 |
| 172Heat — | Ryan Gordon | University at Buffalo Swim Tea | 0.55 | 58.42 |
| 173Heat — | Isaac Gwin | Nitro Swimming | 0.72 | 58.43 |
| 174Heat — | Brendan Feehery | Lakeside Aquatic Club | 0.67 | 58.46 |
| 175Heat — | Jordan Ross | SwimAtlanta/MICH | 0.63 | 58.50 |
| 176Heat — | Will Arthur | Louisiana State University | 0.55 | 58.58 |
| 176Heat — | Wade O'Brien | NOVA of Virginia Aquatics, Inc | 0.63 | 58.58 |
| 178Heat — | Nick Hasemann | University of Louisville/ACAD | 0.68 | 58.59 |
| 179Heat — | Ryan Baker | Arlington Aquatic Club | 0.69 | 58.62 |
| 180Heat — | Kevin Lindgren | Martin County Swimming | 0.68 | 58.65 |
| 181Heat — | Thomas Anderson | Irish Aquatics/CAST | 0.68 | 58.82 |
| 182Heat — | Alex McCarter | New York City Aquatic Club | 0.62 | 58.83 |
| 183Heat — | Timothy Ware | Unattached UDEL | 0.64 | 59.50 |
| —Heat — | Andrew Appleby | Ohio State University/CFVY | 0.61 | DQ |
| —Heat — | Ryan Dudzinski | Stanford Swimming/USCS | — | |