| 1Heat — | Caeleb Dressel | Bolles School Sharks/FLOR | 0.64 | 21.76 |
| 2Heat — | Anthony Ervin | Unattached MAC/UCB | 0.69 | 21.80 |
| 3Heat — | Cullen Jones | SwimMAC Carolina | 0.65 | 21.84 |
| 4Heat — | Nathan Adrian | California Aquatics | 0.64 | 21.96 |
| 5Heat — | Jimmy Feigen | New York Athletic Club/MAC | 0.62 | 22.11 |
| 6Heat — | Paul Powers | Univ. Of Michigan Swim Team/SAC | 0.67 | 22.22 |
| 7Heat — | Michael Chadwick | U. of Missouri/MAC | 0.70 | 22.24 |
| 8Heat — | William Copeland | Rockbridge Storm Swim Team | 0.64 | 22.25 |
| 9Heat — | Michael Andrew | Indie Swimming | 0.71 | 22.27 |
| 10Heat — | Payton Sorenson | Brigham Young University Swim/MAC | 0.67 | 22.29 |
| 11Heat — | Josh Schneider | New York Athletic Club | 0.60 | 22.32 |
| 12Heat — | John Murray | Alamo Area Aquatic Association/UT | 0.63 | 22.34 |
| 13Heat — | Ryan Held | NC State University | 0.65 | 22.37 |
| 14Heat — | Dillon Virva | King Aquatic Club/UNLV | 0.63 | 22.40 |
| 15Heat — | Bowen Becker | University of Minnesota/SAND | 0.72 | 22.45 |
| 15Heat — | David Williams | Wolfpack Elite | 0.72 | 22.45 |
| 17Heat — | Caleb Weir | Wolfpack Elite | 0.65 | 22.53 |
| 18Heat — | Tim Squires | Austin Swim Club | 0.64 | 22.57 |
| 19Heat — | Bradley Deborde | Gator Swim Club | 0.65 | 22.58 |
| 20Heat — | Luke Pechmann | Canyons Aquatic Club | 0.66 | 22.59 |
| 20Heat — | Drew Modrov | Swimmin | 0.69 | 22.59 |
| 22Heat — | Kyle Decoursey | Tennessee Aquatics/ZSC | 0.74 | 22.64 |
| 23Heat — | Derek Toomey | Badger Swim Club Inc/TROJ | 0.64 | 22.65 |
| 24Heat — | Wyatt Ubellacker | Harvard University | 0.71 | 22.67 |
| 25Heat — | Zach Apple | Auburn University Swim Club | 0.65 | 22.69 |
| 26Heat — | Nick Soedel | Swim Utah | 0.61 | 22.76 |
| 27Heat — | Michael Trice | SwimAtlanta/UGA | 0.71 | 22.85 |
| 28Heat — | Travis Lauri | Fairfax Foxes Swimming | 0.69 | 22.89 |
| 28Heat — | Kasey Foley | Team Rebel Aquatics/TRA | 0.72 | 22.89 |
| 30Heat — | Mark Weber | SwimMAC Carolina/FSU | 0.63 | 22.90 |
| 31Heat — | Jack Franzman | Zionsville Swim Club | 0.69 | 22.92 |
| 31Heat — | Ollie Smith | Eagle Swim Club | 0.68 | 22.92 |
| 31Heat — | David Lambert | NTC Aquatics | 0.59 | 22.92 |
| 34Heat — | Kyle Darmody | Auburn University Swim Club/MAC | 0.68 | 22.93 |
| 34Heat — | Griffin Schumacher | Irvine Novaquatics/HARV | 0.68 | 22.93 |
| 36Heat — | Blake Pieroni | Indiana University/DUNE | 0.64 | 22.94 |
| 36Heat — | Ian Ho | H2okie Aquatics/VTECH | 0.61 | 22.94 |
| 36Heat — | Fiala Cj | Unattached | 0.66 | 22.94 |
| 39Heat — | Ryan Bubb | Heartland Aquatics | 0.67 | 22.97 |
| 39Heat — | Kyle Robrock | University of Denver Hilltoppe/DU | 0.66 | 22.97 |
| 41Heat — | Chatham Dobbs | Tucson Ford Dealers Aquatics/ARIZ | 0.67 | 22.98 |
| 41Heat — | Adam Powell | Unattached | 0.70 | 22.98 |
| 43Heat — | Ryan Hoffer | Scottsdale Aquatic Club | 0.69 | 23.01 |
| 44Heat — | Jason McCormick | Club Seminole | 0.72 | 23.02 |
| 45Heat — | Robert Howard | University Of Alabama Swim Clu | 0.68 | 23.03 |
| 46Heat — | Matt McHugh | Ohio State University | 0.67 | 23.04 |
| 47Heat — | Drew Kibler | Carmel Swim Club | 0.61 | 23.05 |
| 48Heat — | Brett Ringgold | North Texas Nadadores/UT | 0.77 | 23.06 |
| 49Heat — | Eric Knight | SwimMAC Carolina | 0.69 | 23.07 |
| 49Heat — | Chad Mylin | Club Seminole | 0.67 | 23.07 |
| 51Heat — | Cannon Clifton | Wisconsin Aquatics | 0.71 | 23.10 |
| 51Heat — | Michael Jensen | Upper Dublin Aquatic Club | 0.70 | 23.10 |
| 51Heat — | Paul O'Hara | Harvard University/NCAP | 0.66 | 23.10 |
| 51Heat — | Daniel Kanorr | Badger Swim Club Inc/IND | 0.69 | 23.10 |
| 55Heat — | Kevin Ferguson | The Olympic Club | 0.72 | 23.11 |
| 55Heat — | Shayne Fleming | De Anza Cupertino Aquatics | 0.64 | 23.11 |
| 57Heat — | Tate Jackson | Longhorn Aquatics/UT | 0.70 | 23.12 |
| 57Heat — | Jeff Garnier | Stanford Swimming | 0.72 | 23.12 |
| 59Heat — | Daniel Krueger | McFarland Spartan Sharks | 0.78 | 23.13 |
| 60Heat — | David Sweeney | University of Pittsburgh | 0.73 | 23.14 |
| 61Heat — | Patrick Park | Pitchfork Aquatics/ASU | 0.64 | 23.15 |
| 61Heat — | Mossimo Chavez | Ohio State University | 0.61 | 23.15 |
| 63Heat — | Justin Ress | Marlins of Raleigh/NCSU | 0.68 | 23.16 |
| 64Heat — | Wade Allen | Gaucho Aquatics | 0.66 | 23.17 |
| 65Heat — | Michael Duderstadt | Auburn University Swim Club/PCST | 0.64 | 23.19 |
| 66Heat — | Paul Fair | University of Minnesota | 0.67 | 23.20 |
| 66Heat — | Michael Arnold | Chattahoochee Gold Swim Club | 0.65 | 23.20 |
| 66Heat — | Tommy Thetford | Northern KY Clippers Swimming | 0.66 | 23.20 |
| 66Heat — | Spencer DeShon | Stanford Swimming/MVN | 0.63 | 23.20 |
| 70Heat — | Adam Johnston | Boilermaker Aquatics/PUR | 0.72 | 23.21 |
| 70Heat — | Ryan Torie | Germantown Academy Aquatic Cl | 0.77 | 23.21 |
| 70Heat — | Dean Farris | Metro Atlanta Aquatic Club | 0.69 | 23.21 |
| 73Heat — | James Jones | Nation's Capital Swim Club | 0.65 | 23.22 |
| 74Heat — | Brandon Crabtree | San Diego YMCA | 0.67 | 23.23 |
| 74Heat — | Eric Bruck | Kentucky Aquatics | 0.71 | 23.23 |
| 76Heat — | Jason Chen | Unattached | 0.66 | 23.24 |
| 77Heat — | Connor Black | Unattached | 0.69 | 23.25 |
| 77Heat — | Zach Hayden | Club Wolverine | 0.66 | 23.25 |
| 77Heat — | Mark McGlaughlin | Iowa Flyers Swim Club | 0.69 | 23.25 |
| 80Heat — | Joe Bonk | YMCA of the Triangle Area Swim/NCS | 0.68 | 23.26 |
| 81Heat — | Alexander Forbes | Central Florida Aquatics | 0.69 | 23.28 |
| 81Heat — | Will Davis | Bolles School Sharks | 0.62 | 23.28 |
| 81Heat — | Chris Szekely | Army West Point | 0.71 | 23.28 |
| 81Heat — | Scott Johnson | Marlins of Raleigh/NCSU | 0.71 | 23.28 |
| 85Heat — | Dunne Pj | City of Mobile Swim Associatio/UT | 0.70 | 23.29 |
| 86Heat — | John Wagner | Rice Aquatics/TAMU | 0.63 | 23.31 |
| 86Heat — | Brent Benedict | H2okie Aquatics/VTECH | 0.62 | 23.31 |
| 88Heat — | Danny Tucker | Boilermaker Aquatics/PUR | 0.71 | 23.32 |
| 89Heat — | Trevor Carroll | University of Louisville/Stars | 0.61 | 23.33 |
| 89Heat — | Renny Richmond | Tucson Ford Dealers Aquatics | 0.68 | 23.33 |
| 91Heat — | Matt Lockman | Cavalier Swimming | 0.65 | 23.34 |
| 91Heat — | Oliver Patrouch | Indiana University/BAD | 0.66 | 23.34 |
| 91Heat — | Vinny Tafuto | Unattached MICH | 0.65 | 23.34 |
| 91Heat — | Christopher Peterson | Orinda Aquatics/UCSB | 0.61 | 23.34 |
| 95Heat — | Jeremy Nichols | Unattached RACE/UT | 0.75 | 23.37 |
| 95Heat — | Sam Lorentz | Indiana University | 0.68 | 23.37 |
| 95Heat — | Eric Schultz | University of Pennsylvania | 0.69 | 23.37 |
| 98Heat — | Jamal Willis | Penn Charter Aquatic Club | 0.66 | 23.38 |
| 99Heat — | Ian Apple | Club Seminole | 0.66 | 23.39 |
| 99Heat — | John Long | Unattached QU/QU | 0.66 | 23.39 |
| 101Heat — | Nathan Lile | Gardner-Webb Univ Aquatics | 0.67 | 23.40 |
| 102Heat — | Andrew Marsh | Club Mountaineer Aquatics/WVU | 0.66 | 23.41 |
| 103Heat — | Jesse Novak | Rowan University | 0.74 | 23.42 |
| 103Heat — | Jack Blake | Scottsdale Aquatic Club | 0.68 | 23.42 |
| 105Heat — | Noah Busch | University of Minnesota/MTKA | 0.67 | 23.45 |
| 106Heat — | Christian Aragona | U. of Missouri/TCAY | 0.69 | 23.46 |
| 106Heat — | Thomas Dillinger | Greenwich YWCA/PENN | 0.68 | 23.46 |
| 106Heat — | Sam Disette | Kansas City Swim Academy | 0.64 | 23.46 |
| 109Heat — | Colin Eaton | Mount Hood Aquatics | 0.59 | 23.48 |
| 109Heat — | Kyle White | Wyoming Aquatics/UWY | 0.65 | 23.48 |
| 111Heat — | Chuck Destro | Unattached | 0.67 | 23.49 |
| 111Heat — | Jackson Halsmer | University Of Iowa | 0.68 | 23.49 |
| 113Heat — | Tyler Harper | Stingrays/LSU | 0.63 | 23.50 |
| 113Heat — | Luke Mankus | U. of Missouri/MTRO | 0.66 | 23.50 |
| 115Heat — | Ryan Barsanti | Wisconsin Aquatics/TERA | 0.76 | 23.51 |
| 116Heat — | Daniel Kelly | Team Millennium/BC | 0.71 | 23.52 |
| 117Heat — | Andrew Saeta | The Olympic Club | 0.74 | 23.53 |
| 117Heat — | Hemmingsen Rj | University Of Iowa | 0.66 | 23.53 |
| 117Heat — | Brian Donatelli | Lyons Swim Club | 0.65 | 23.53 |
| 120Heat — | Noah Lucas | University of North Dakota | 0.66 | 23.54 |
| 120Heat — | Justin Glanda | Club Wolverine | 0.63 | 23.54 |
| 120Heat — | Cobe Garcia | Kentucky Aquatics/AAAA | 0.65 | 23.54 |
| 123Heat — | Darian Townsend | YMCA Westside Silver Fins | 0.70 | 23.55 |
| 124Heat — | Mike Dugan | Buffalo Area Aquatic Club | 0.68 | 23.56 |
| 124Heat — | Kevin Musterait | Ohio State University | 0.74 | 23.56 |
| 124Heat — | Justin White | Team Millennium/Lehigh | 0.69 | 23.56 |
| 127Heat — | Gage Kohner | Northwestern University ST/SAS | 0.71 | 23.57 |
| 128Heat — | Hunter Hoffman | Anderson Comm Aquatics Club | 0.66 | 23.58 |
| 128Heat — | Sebastian Lutz | Harvard University | 0.64 | 23.58 |
| 128Heat — | Ryan Boraski | Kingfish Swimming/KSC | 0.65 | 23.58 |
| 131Heat — | Albert Gwo | Palo Alto Stanford Aquatics | 0.64 | 23.60 |
| 131Heat — | Jeremy Parker | Greater Tampa Swimming Associa | 0.62 | 23.60 |
| 133Heat — | Sid Farber | Portland Aquatic Club | 0.69 | 23.61 |
| 134Heat — | Jordan Dahle | Air Force Academy | 0.68 | 23.62 |
| 134Heat — | Luke Burton | SwimMAC Carolina/DAV | 0.75 | 23.62 |
| 136Heat — | Jason Deana | Wolfpack Elite/NCS | 0.71 | 23.63 |
| 137Heat — | Stanley Wong | NYS Aquatics Inc | 0.64 | 23.64 |
| 137Heat — | Kent Olsen-Stavrakas | Colorado Stars | 0.57 | 23.64 |
| 139Heat — | Connor Stirling | Brigham Young University Swim/LTAZ | 0.71 | 23.65 |
| 140Heat — | Lucas Popp | North Carolina Aquatic Club | 0.69 | 23.69 |
| 141Heat — | Billy Beard | Upper Main Line YMCA | 0.64 | 23.70 |
| 141Heat — | Eddy Zawatski | CYC Rally Aquatic Club | 0.67 | 23.70 |
| 143Heat — | Blaise Vera | Mississippi Makos Swim Team | 0.62 | 23.73 |
| 143Heat — | John Wesley | SwimAtlanta/UGA | 0.68 | 23.73 |
| 145Heat — | Justin Hill | SwimStrongsville | 0.69 | 23.74 |
| 146Heat — | Miles Mackenzie | Unattached | 0.67 | 23.75 |
| 147Heat — | Luke Hemmingsen | Greater Omaha Aquatics | 0.67 | 23.76 |
| 147Heat — | Eric McGinnis | Trinity Prep Aquatics | 0.58 | 23.76 |
| 149Heat — | Marcin Glebocki | Northland United Swim Team | 0.64 | 23.77 |
| 150Heat — | Hunter Doerr | Colorado Springs Swim Team | 0.64 | 23.79 |
| 150Heat — | Cole Cogswell | Canyons Aquatic Club | 0.61 | 23.79 |
| 150Heat — | Stephen Culberson | Streamline Aquatics/TU | 0.67 | 23.79 |
| 153Heat — | David Goll | Life Time Arizona Swim Team | 0.68 | 23.80 |
| 153Heat — | Daryl Turner | University of Minnesota/ACES | 0.72 | 23.80 |
| 155Heat — | Eric Strickland | Fowler Family YMCA | 0.66 | 23.85 |
| 156Heat — | Zach Wagner | King Aquatic Club | 0.65 | 23.88 |
| 157Heat — | Matthew Margritier | Unattached TST/UMIZ | 0.63 | 23.99 |
| 158Heat — | Michael Saco | Miami Swimming | 0.74 | 24.01 |
| 158Heat — | Kanoa Kaleoaloha | Kamehameha Swim Club/SEM | 0.67 | 24.01 |
| 160Heat — | Brian Anderson | Unattached | 0.70 | 24.02 |
| 161Heat — | Kevin Dang | King Aquatic Club | 0.65 | 24.03 |
| 162Heat — | Michael Seaberg | Duke University/NAAC | 0.63 | 24.05 |
| 163Heat — | Colin Macquarrie | University of North Dakota | 0.62 | 24.08 |
| 164Heat — | Orion Young | Team Santa Monica | 0.68 | 24.19 |
| 165Heat — | Jacob Phillips | Unattached JTSC | 0.71 | 24.90 |
| —Heat — | Austin Hirstein | Tennessee Aquatics | 0.65 | DQ |
| —Heat — | Seth Stubblefield | California Aquatics | — | |
| —Heat — | Troy Tillman | Tennessee Aquatics | — | |
| —Heat — | Matt Grevers | Tucson Ford Dealers Aquatics | — | |
| —Heat — | Eric Williams | Boca Raton Swim Team | — | NS |
| —Heat — | Alex Ngan | Golden Rebels | — | NS |
| —Heat — | Alex Gray | University Of Alabama Swim Clu | — | NS |