| 1Heat — | —Sarah Henry | Aggie Swim Club | 0.78 | 4:36.93 |
| 2Heat — | —Elizabeth Beisel | Bluefish Swim Club/FLOR | 0.67 | 4:37.61 |
| 3Heat — | —Bethany Galat | Texas A & M University/SBSC | 0.60 | 4:38.39 |
| 4Heat — | —Maya DiRado | Stanford Swimming/SRN | 0.68 | 4:38.54 |
| 5Heat — | —Caitlin Leverenz | California Aquatics | 0.70 | 4:39.55 |
| 6Heat — | —Madisyn Cox | Lubbock Swim Club/UT | 0.72 | 4:40.97 |
| 7Heat — | —Kate Mills | SwimMAC Carolina | 0.62 | 4:41.40 |
| 8Heat — | —Lindsey Clary | Ohio State University | 0.75 | 4:42.04 |
| 9Heat — | —Ella Eastin | Stanford Swimming/SoCal | 0.72 | 4:42.08 |
| 10Heat — | —Brooke Zeiger | University of Minnesota/CRIM | 0.77 | 4:42.45 |
| 11Heat — | —Cammile Adams | SwimMAC Carolina | 0.74 | 4:42.61 |
| 12Heat — | —Emily Cameron | Athens Bulldog Swim Club/UGA | 0.68 | 4:42.73 |
| 13Heat — | —Mia Nonnenberg | University Of Alabama Swim Clu | 0.65 | 4:43.87 |
| 14Heat — | —Emma Barksdale | Davis Arden Racing Team | 0.72 | 4:44.80 |
| 15Heat — | —Brooke Forde | Lakeside Swim Team | 0.77 | 4:46.59 |
| 16Heat — | —Meaghan Raab | Nashville Aquatic Club/UGA | 0.75 | 4:48.77 |
| 17Heat — | —Hannah Kastigar | Aberdeen Swim Club | 0.72 | 4:48.78 |
| 18Heat — | —Margaret Aroesty | Long Island Aquatic Club | 0.63 | 4:49.07 |
| 19Heat — | —Melissa Postoll | Northwestern University ST/RAYS | 0.75 | 4:49.57 |
| 20Heat — | —Christin Rockway | Tampa Bay Aquatics | 0.76 | 4:49.63 |
| 21Heat — | —Kaitlyn Jones | Cavalier Swimming | 0.71 | 4:50.18 |
| 22Heat — | —Kay Sargent | Victor Swim Club | 0.67 | 4:50.22 |
| 23Heat — | —Kathryn Painter | Kentucky Aquatics | 0.73 | 4:50.32 |
| 24Heat — | —Madison Homovich | Marlins of Raleigh | 0.66 | 4:50.36 |
| 25Heat — | —Hailey Houck | Sandpipers Of Nevada | 0.77 | 4:50.41 |
| 26Heat — | —Lisa Bratton | Texas A & M University/TCCC | 0.66 | 4:50.50 |
| 27Heat — | —Sammie Burchill | Carmel Swim Club | 0.68 | 4:50.55 |
| 28Heat — | —Sharli Brady | U. of Missouri/CLPR | 0.76 | 4:50.97 |
| 29Heat — | —Alexis Yager | Academy Bullets Swim Club | 0.82 | 4:51.32 |
| 30Heat — | —Caty Hulsey | North Carolina Aquatic Club | 0.76 | 4:51.52 |
| 31Heat — | —Camryn Toney | Texas Ford Aquatics | 0.73 | 4:51.61 |
| 32Heat — | —Mckenna Meyer | Reno Aquatic Club | 0.78 | 4:51.71 |
| 33Heat — | —Sam Lisy | Indiana University/STRS | 0.81 | 4:51.85 |
| 34Heat — | —Kim Williams | Stanford Swimming/BC | 0.81 | 4:52.21 |
| 35Heat — | —Megan Brown | Greenwood Swimming/FSU | 0.76 | 4:52.41 |
| 36Heat — | —Lindsay Looney | Metroplex Aquatics | 0.78 | 4:52.78 |
| 36Heat — | —Kaitlin Pawlowicz | Nation's Capital Swim Club/UT | 0.77 | 4:52.78 |
| 38Heat — | —Marah Pugh | University of Louisville | 0.61 | 4:52.88 |
| 39Heat — | —Celina Li | California Aquatics/PLS | 0.65 | 4:53.21 |
| 40Heat — | —Annie Zhu | Athens Bulldog Swim Club/UGA | 0.75 | 4:53.29 |
| 41Heat — | —Rachael Bradford-Feldman | University of Louisville/SAS | 0.76 | 4:53.33 |
| 42Heat — | —Olivia Johnson | Fishers Area Swimming Tigers | 0.65 | 4:53.53 |
| 43Heat — | —Abby Fisher | North Carolina Aquatic Club | 0.71 | 4:53.57 |
| 44Heat — | —Bailey Pressey | Indiana University | 0.75 | 4:53.65 |
| 45Heat — | —Regan Barney | Unattached TXLA/TXLA | 0.78 | 4:53.69 |
| 45Heat — | —Hannah Burns | Gator Swim Club | 0.74 | 4:53.69 |
| 47Heat — | —Katie Trace | Upper Arlington Swim Club | 0.72 | 4:53.85 |
| 48Heat — | —Alex Sumner | Suburban Seahawks Club | 0.77 | 4:53.90 |
| 49Heat — | —Meghan Small | York YMCA | 0.76 | 4:54.05 |
| 50Heat — | —Carley Lowe | Highlander Aquatic Club | 0.72 | 4:54.14 |
| 50Heat — | —Eleanor Matheson | Flatiron Athletic Club | 0.74 | 4:54.14 |
| 52Heat — | —Chelsie Miller | Kansas Swimming/Fleet | 0.75 | 4:54.30 |
| 53Heat — | —Evie Pfeifer | CSP Tideriders | 0.76 | 4:54.42 |
| 54Heat — | —Carly Cummings | Badger Swim Club Inc | 0.72 | 4:54.51 |
| 55Heat — | —Ella Nelson | Nashville Aquatic Club | 0.73 | 4:54.57 |
| 56Heat — | —Chenoa Devine | Davis Arden Racing Team | 0.75 | 4:55.12 |
| 57Heat — | —Kelly Fertel | Gulliver Swim Club | 0.77 | 4:55.50 |
| 58Heat — | —Vanessa Pearl | Metroplex Aquatics | 0.75 | 4:55.52 |
| 59Heat — | —Lizzie Zeiger | Crimson Aquatics | 0.76 | 4:55.73 |
| 60Heat — | —Nora McCullagh | SwimMAC Carolina/UT | 0.80 | 4:55.78 |
| 61Heat — | —Haley Rowley | University of Denver Hilltoppe/CSU | 0.64 | 4:55.79 |
| 62Heat — | —Cassidy Stein | University of Denver Hilltoppe | 0.76 | 4:55.82 |
| 63Heat — | —Erin Earley | Hopkins Mariner Swim Team | 0.68 | 4:55.84 |
| 64Heat — | —Destiny Nelson | Texas Ford Aquatics/Yale | 0.77 | 4:55.88 |
| 65Heat — | —Kaitlyn Swinney | Rice Aquatics/FCST | 0.73 | 4:55.97 |
| 66Heat — | —Leah Stevens | Stanford Swimming/LAKE | 0.70 | 4:56.19 |
| 67Heat — | —Molly Kowal | Jersey Wahoos | 0.85 | 4:56.22 |
| 68Heat — | —Maddie Myers | University of Denver Hilltoppe/DU | 0.72 | 4:56.26 |
| 68Heat — | —Ally McHugh | Penn State University/SSC | 0.78 | 4:56.26 |
| 70Heat — | —Hannah Snyder | YMCA of the Triangle Area Swim/TSU | 0.75 | 4:56.38 |
| 71Heat — | —Dannie Dilsaver | Greater Nebraska Swim Team | 0.74 | 4:56.49 |
| 72Heat — | —Kathleen Golding | South Florida Aquatic Club | 0.76 | 4:56.96 |
| 73Heat — | —Jessica Epps | Mission Viejo Nadadores | 0.72 | 4:56.97 |
| 73Heat — | —Jessie Garrison | Razorback Aquatic Club Aquahaw/ARK | 0.62 | 4:56.97 |
| 75Heat — | —Katie Glavinovich | Aquazot Swim Club | 0.75 | 4:57.02 |
| 76Heat — | —Danika Katzer | Coast Guard Blue Dolphins | 0.74 | 4:57.11 |
| 77Heat — | —Isabella Goldsmith | Sandpipers Of Nevada | 0.68 | 4:57.20 |
| 78Heat — | —Jordan Anderson | Swim Utah | 0.70 | 4:57.22 |
| 79Heat — | —Carolyn Johnston | Rockwall Aquatic Center of Exc | 0.67 | 4:57.33 |
| 80Heat — | —Julia Poole | Unattached MOR | 0.76 | 4:57.44 |
| 81Heat — | —Whitney Jorgensen | Sierra Marlins Swim Team/UOP | 0.79 | 4:57.57 |
| 82Heat — | —Megan Byrnes | Nation's Capital Swim Club | 0.82 | 4:57.84 |
| 83Heat — | —Daniela Georges | Tucson Ford Dealers Aquatics/AZOT | 0.78 | 4:57.89 |
| 84Heat — | —Isabel Gormley | Asphalt Green Unified Aquatics | 0.76 | 4:57.97 |
| 85Heat — | —Libby Walker | Kansas Swimming/CSC | 0.82 | 4:58.10 |
| 86Heat — | —Ashlyn Fiorilli | Texas Ford Aquatics | 0.78 | 4:58.17 |
| 87Heat — | —Lauren Abruzzo | Northwestern University ST/TOPS | 0.69 | 4:58.27 |
| 88Heat — | —Maggie D'Innocenzo | Longhorn Aquatics/UT | 0.67 | 4:58.37 |
| 89Heat — | —Sandra Freeman | RallySport Aquatic Club | 0.77 | 4:58.49 |
| 90Heat — | —Sonia Wang | Harvard University/AZOT | 0.68 | 4:58.82 |
| 91Heat — | —Miranda Heckman | Pleasanton Seahawks | 0.77 | 4:58.99 |
| 92Heat — | —Alexis Preski | Academy Bullets Swim Club | 0.67 | 4:59.14 |
| 93Heat — | —Jessie Gvozdas | NOVA of Virginia Aquatics, Inc/UVA | 0.76 | 4:59.32 |
| 94Heat — | —Callie Dickinson | Tide Swimming | 0.74 | 4:59.35 |
| 95Heat — | —Devan Sweeney | SwimAtlanta | 0.75 | 4:59.57 |
| 96Heat — | —Jasmine Margetts | Beach Cities Swimming | 0.74 | 4:59.97 |
| 97Heat — | —Bailey Bonnett | Fox Chapel Killer Whales | 0.68 | 5:00.54 |
| 98Heat — | —Mikki Thompson | Brea Aquatics | 0.71 | 5:00.85 |
| 99Heat — | —Olivia Paoletti | Delaware Swim Team | 0.76 | 5:00.92 |
| 100Heat — | —Charlotte Meyer | Navy Swimming/RMSC | 0.66 | 5:01.04 |
| 101Heat — | —Zoe Gawronska | The Woodlands Swim Team | 0.76 | 5:01.17 |
| 102Heat — | —Erica Sullivan | Sandpipers Of Nevada | 0.76 | 5:01.19 |
| 103Heat — | —Megan Pierce | Shawmut Aquatic Club/WILL | 0.76 | 5:01.60 |
| 104Heat — | —Rachel Politi | Cavalier Swimming | 0.72 | 5:01.69 |
| 105Heat — | —Kate Krolikowski | Aquazot Swim Club | 0.68 | 5:01.73 |
| 106Heat — | —Zoe Thatcher | Auburn University Swim Club/Rays | 0.77 | 5:02.25 |
| 107Heat — | —Emma Muzzy | Virginia Gators | 0.77 | 5:02.65 |
| 107Heat — | —Allie Szekely | Central Bucks Swim Team | 0.76 | 5:02.65 |
| 109Heat — | —Halladay Kinsey | Davis Arden Racing Team | 0.69 | 5:02.78 |
| 110Heat — | —Casey Francis | Penn State University/PSC | 0.75 | 5:02.91 |
| 111Heat — | —Jaden Bellina | Pearland Aquatics | 0.77 | 5:03.05 |
| 112Heat — | —Madison Boswell | Poseidon Swimming Inc/UNC | 0.66 | 5:03.93 |
| 113Heat — | —Mikayla Herich | Northern KY Clippers Swimming | 0.88 | 5:05.16 |
| 114Heat — | —Abby Chin | Upper Arlington Swim Club/UOFL | 0.72 | 5:05.83 |
| 115Heat — | —Sydney Lofquist | Trojan Swim Club/RAYS | 0.79 | 5:06.47 |
| 116Heat — | —Nora Deleske | Golden West Swim Club | 0.76 | 5:07.42 |
| 116Heat — | —Jocelyn Wang | Scottsdale Aquatic Club | 0.65 | 5:07.42 |
| 118Heat — | —Chloe Isleta | Palo Alto Stanford Aquatics | 0.69 | 5:07.75 |
| 119Heat — | —Jessica Antiles | Unattached COL | 0.72 | 5:09.37 |
| —Heat — | —Colleen Callahan | University Of Tennessee/SEA | 0.74 | DQ |
| —Heat — | —Ashley Neidigh | Auburn University Swim Club | 0.79 | DQ |
| —Heat — | —Savanna Faulconer | Y-Spartaquatics Swim Club | 0.76 | DQ |
| —Heat — | —Lauren Edelman | Fishers Area Swimming Tigers | 0.68 | DQ |
| —Heat — | —Hannah Moore | NC State University | 0.71 | DQ |
| —Heat — | —Kristen Romano | Long Island Aquatic Club | 0.67 | DQ |
| —Heat — | —Verity Abel | Duke University/WYW | 0.65 | DQ |
| —Heat — | —Solie Laughlin | Buenaventura Swim Club/UCD | 0.73 | DQ |
| —Heat — | —Tara Halsted | Davis Arden Racing Team | — | |
| —Heat — | —Bailey Nero | Auburn University Swim Club/FAST | — | |