| 1Heat — | Missy Franklin | Colorado Stars/Cal | 0.63 | 2:09.69 |
| 2Heat — | Maya DiRado | Stanford Swimming/SRN | 0.58 | 2:09.76 |
| 3Heat — | Elizabeth Beisel | Bluefish Swim Club/FLOR | 0.59 | 2:10.01 |
| 4Heat — | Danielle Galyer | Kentucky Aquatics/YSSC | 0.55 | 2:10.35 |
| 5Heat — | Bridgette Alexander | Kentucky Aquatics | 0.63 | 2:10.90 |
| 6Heat — | Eva Merrell | Aquazot Swim Club | 0.66 | 2:11.02 |
| 7Heat — | Tasija Karosas | Longhorn Aquatics/UT | 0.64 | 2:11.23 |
| 8Heat — | Erin Voss | Bluefish Swim Club/WAC | 0.69 | 2:11.24 |
| 9Heat — | Melissa Postoll | Northwestern University ST/RAYS | 0.61 | 2:11.67 |
| 10Heat — | Alex Walsh | Nashville Aquatic Club | 0.70 | 2:11.68 |
| 11Heat — | Amy Bilquist | California Aquatics/CSC | 0.70 | 2:11.77 |
| 12Heat — | Lisa Bratton | Texas A & M University/TCCC | 0.65 | 2:11.92 |
| 13Heat — | Clara Smiddy | Univ. Of Michigan Swim Team/AKS | 0.67 | 2:11.98 |
| 14Heat — | Ally Howe | Stanford Swimming/PASA | 0.68 | 2:12.08 |
| 15Heat — | Callie Dickinson | Tide Swimming | 0.57 | 2:12.14 |
| 16Heat — | Quinn Carrozza | Longhorn Aquatics/UT | 0.76 | 2:12.19 |
| 17Heat — | Kaitlin Harty | YMCA of the North Shore | 0.62 | 2:12.21 |
| 18Heat — | Rhyan White | Wasatch Front Fish Market | 0.67 | 2:12.36 |
| 19Heat — | Janet Hu | Stanford Swimming/NCAP | 0.53 | 2:12.36 |
| 20Heat — | Alex Sumner | Suburban Seahawks Club | 0.57 | 2:12.41 |
| 21Heat — | Asia Seidt | Lakeside Swim Team | 0.61 | 2:12.45 |
| 22Heat — | Grace Ariola | Waves Bloomington/Normal Y S.T | 0.58 | 2:12.58 |
| 23Heat — | Lucie Nordmann | Magnolia Aquatic Club | 0.64 | 2:12.71 |
| 24Heat — | Hali Flickinger | Athens Bulldog Swim Club/UGA | 0.68 | 2:12.93 |
| 25Heat — | Megan Moroney | Cavalier Swimming/SAS | 0.58 | 2:13.12 |
| 26Heat — | Emma Seiberlich | Phoenixville YMCA | 0.74 | 2:13.13 |
| 27Heat — | Melanie Klaren | California Aquatics | 0.64 | 2:13.15 |
| 28Heat — | Alice Treuth | Irish Aquatics/ND | 0.71 | 2:13.19 |
| 29Heat — | Jen Marrkand | Cavalier Swimming | 0.60 | 2:13.28 |
| 30Heat — | Madison White | UCLA Swimming | 0.65 | 2:13.40 |
| 31Heat — | Cameron McHugh | Tucson Ford Dealers Aquatics/CFSC | 0.62 | 2:13.52 |
| 32Heat — | Claire Brandt | Texas A & M University | 0.65 | 2:13.78 |
| 33Heat — | Regan Smith | Riptide | 0.60 | 2:14.20 |
| 34Heat — | Paige Madden | City of Mobile Swim Associatio | 0.67 | 2:14.29 |
| 35Heat — | Sophie Krivokapic-Zhou | United States | 0.67 | 2:14.30 |
| 36Heat — | Kyra Sarazen | Irish Aquatics/ND | 0.58 | 2:14.34 |
| 37Heat — | Kaitlyn Jones | Cavalier Swimming | 0.69 | 2:14.56 |
| 38Heat — | Hannah Weiss | Trojan Swim Club/USC | 0.64 | 2:14.72 |
| 39Heat — | Solie Laughlin | Buenaventura Swim Club/UCD | 0.64 | 2:14.78 |
| 40Heat — | Madison Homovich | Marlins of Raleigh | 0.67 | 2:15.09 |
| 41Heat — | Hellen Moffitt | North Carolina Aquatic Club | 0.63 | 2:15.10 |
| 42Heat — | Alyssa Yambor-Maul | Area Tallahassee Aquatic Club | 0.64 | 2:15.15 |
| 43Heat — | Bonnie Brandon | Tucson Ford Dealers Aquatics/MACS | 0.73 | 2:15.16 |
| 44Heat — | Hannah Stevens | U. of Missouri/GCST | 0.63 | 2:15.29 |
| 45Heat — | Sammie Burchill | Carmel Swim Club | 0.59 | 2:15.30 |
| 45Heat — | Bayley Stewart | Fort Collins Area Swim Team | 0.65 | 2:15.30 |
| 47Heat — | Erin Earley | Hopkins Mariner Swim Team | 0.73 | 2:15.31 |
| 48Heat — | Kacey Oberlander | York YMCA | 0.67 | 2:15.35 |
| 49Heat — | Marcie Maguire | Ridgefield Aquatic Club | 0.66 | 2:15.53 |
| 50Heat — | Katharine Berkoff | Missoula Aquatic Club | 0.69 | 2:15.55 |
| 51Heat — | Meghan Small | York YMCA | 0.69 | 2:15.59 |
| 52Heat — | Abi Wilder | Bolles School Sharks | 0.62 | 2:15.60 |
| 53Heat — | Elizabeth Pelton | California Aquatics/T2 | 0.66 | 2:15.69 |
| 54Heat — | Kaitlyn Ferrara | Ohio State University | 0.64 | 2:16.15 |
| 55Heat — | Emma Schanz | UCLA Swimming/SWAT | 0.65 | 2:16.16 |
| 56Heat — | Erin Oeltjen | Club Huskers/MAC | 0.64 | 2:16.20 |
| 57Heat — | Eireni Moshos | Nation's Capital Swim Club | 0.70 | 2:16.30 |
| 58Heat — | Destiny Nelson | Texas Ford Aquatics/Yale | 0.69 | 2:16.31 |
| 59Heat — | Emily Slabe | North Carolina Aquatic Club | 0.68 | 2:16.32 |
| 60Heat — | Chloe Hicks | Machine Aquatics | 0.63 | 2:16.33 |
| 61Heat — | Elise Forzley | Fort Collins Area Swim Team | 0.59 | 2:16.34 |
| 62Heat — | Lindsay Morrow | Wolfpack Elite/NCS | 0.58 | 2:16.40 |
| 63Heat — | Stephanie Nelson | SwimMAC Carolina | 0.67 | 2:16.56 |
| 64Heat — | Kristen Romano | Long Island Aquatic Club | 0.62 | 2:16.63 |
| 65Heat — | Lindsay Temple | Princeton University Swim Team/SVY | 0.64 | 2:16.69 |
| 66Heat — | Ali Galyer | Y-Spartaquatics Swim Club | 0.66 | 2:16.75 |
| 67Heat — | Haley Rowley | University of Denver Hilltoppe/CSU | 0.56 | 2:16.80 |
| 68Heat — | Hanni Leach | Trojan Swim Club/USC | 0.66 | 2:16.83 |
| 69Heat — | Keaton Blovad | Tualatin Hills Swim Club | 0.64 | 2:16.87 |
| 70Heat — | Allie Szekely | Central Bucks Swim Team | 0.68 | 2:16.90 |
| 71Heat — | Katie Glavinovich | Aquazot Swim Club | 0.66 | 2:17.03 |
| 72Heat — | Maddie Hess | Duke University/BW | 0.64 | 2:17.04 |
| 72Heat — | Julia Cook | Aggie Swim Club | 0.75 | 2:17.04 |
| 72Heat — | Sara Metzsch | Tualatin Hills Swim Club | 0.61 | 2:17.04 |
| 75Heat — | Christin Rockway | Tampa Bay Aquatics | 0.68 | 2:17.06 |
| 76Heat — | Evie Pfeifer | CSP Tideriders | 0.71 | 2:17.08 |
| 76Heat — | Becca Postoll | Univ. Of Michigan Swim Team/MAC | 0.64 | 2:17.08 |
| 78Heat — | Lexie Lupton | Texas A & M University/Eagle | 0.59 | 2:17.12 |
| 78Heat — | Camryn Forbes | Butler Y Aquatic Club | 0.56 | 2:17.12 |
| 80Heat — | Cammile Adams | SwimMAC Carolina | 0.69 | 2:17.30 |
| 81Heat — | Ruby Martin | Iowa Flyers Swim Club | 0.68 | 2:17.31 |
| 82Heat — | Courtney Mykkanen | Irvine Novaquatics | 0.67 | 2:17.69 |
| 82Heat — | Christie Chong | Pleasanton Seahawks | 0.66 | 2:17.69 |
| 84Heat — | Morgan McCormick | Unattached | 0.58 | 2:17.70 |
| 85Heat — | Kayla Churman | Kentucky Aquatics | 0.64 | 2:17.79 |
| 86Heat — | Kate Moore | Unattached MOR | 0.64 | 2:17.80 |
| 87Heat — | Kendall Shields | Austin Swim Club | 0.66 | 2:17.83 |
| 88Heat — | Amanda Smith | Northern KY Clippers Swimming | 0.56 | 2:17.86 |
| 89Heat — | Morgan Liberto | Columbia Aquatics Association | 0.70 | 2:17.88 |
| 89Heat — | Taylor McCoy | Gold Medal Swim Club | 0.63 | 2:17.88 |
| 91Heat — | Catherine Ladd | Santa Clara Swim Club/Cal | 0.61 | 2:17.92 |
| 92Heat — | Sarah Welch | YMCA of the North Shore | 0.64 | 2:18.29 |
| 93Heat — | Shelly Drozda | Indiana University/CUDA | 0.65 | 2:18.33 |
| 94Heat — | Tevyn Waddell | Mitchell Aquatic Club | 0.69 | 2:18.37 |
| 94Heat — | Emily Meilus | Machine Aquatics | 0.65 | 2:18.37 |
| 96Heat — | Sydney Sell | Lakeside Swim Team/FLOR | 0.63 | 2:18.52 |
| 97Heat — | Sarah Hitchens | North Carolina Aquatic Club | 0.64 | 2:18.70 |
| 98Heat — | Kate Hazlett | Greenwich YWCA Dolphins Swm Tm | 0.59 | 2:18.72 |
| 99Heat — | Alexis Mitcheltree | Eastern Michigan University/ASC | 0.60 | 2:18.81 |
| 100Heat — | Ashley Boddiford | Blue Dolfins | 0.77 | 2:18.84 |
| 101Heat — | Cassie Kalisz | North Baltimore Aquatic Club | 0.70 | 2:18.85 |
| 102Heat — | Olivia Bray | Virginia Gators | 0.62 | 2:18.91 |
| 103Heat — | Marissa Engel | Cyclone Swim Club | 0.68 | 2:19.20 |
| 104Heat — | Rachel Matsumura | Indiana University/LAKE | 0.61 | 2:19.22 |
| 105Heat — | Elli Ferrin | Bend Swim Club | 0.65 | 2:19.30 |
| 106Heat — | Sophie Sorenson | Ames Cyclone Aquatics Club | 0.70 | 2:19.32 |
| 107Heat — | Katie Kelsoe | University Of Alabama Swim Clu | 0.60 | 2:19.40 |
| 108Heat — | Claire Adams | Carmel Swim Club | 0.66 | 2:19.43 |
| 109Heat — | Karling Hemstreet | Nitro Swimming | 0.61 | 2:19.50 |
| 110Heat — | Olivia Jameson | North Shore Swim Club | 0.64 | 2:19.56 |
| 111Heat — | Sophie Skinner | Northern KY Clippers Swimming | 0.73 | 2:19.65 |
| 112Heat — | Rebecca Baxley | Neptune Swimming/UT | 0.64 | 2:19.83 |
| 113Heat — | Lauren Meeker | NOVA of Virginia Aquatics, Inc | 0.71 | 2:19.85 |
| 113Heat — | Erin Falconer | Auburn University Swim Club/PAC | 0.65 | 2:19.85 |
| 115Heat — | Regan Barney | Unattached TXLA/TXLA | 0.61 | 2:19.96 |
| 116Heat — | Remedy Rule | Longhorn Aquatics/UT | 0.61 | 2:20.02 |
| 117Heat — | Lauryn Parrish | Fishers Area Swimming Tigers | 0.60 | 2:20.10 |
| 118Heat — | Ashlyn Schoof | Schroeder YMCA Swim Team | 0.60 | 2:20.33 |
| 119Heat — | Kristen Keifer | Kentucky Aquatics/LAK | 0.48 | 2:20.44 |
| 120Heat — | Zoe Gawronska | The Woodlands Swim Team | 0.78 | 2:20.50 |
| 121Heat — | Ashley Leclair | University of Louisville | 0.62 | 2:20.55 |
| 122Heat — | Lauren Poole | North Baltimore Aquatic Club | 0.62 | 2:20.99 |
| 123Heat — | Megan Byrnes | Nation's Capital Swim Club | 0.62 | 2:21.07 |
| 124Heat — | Darby Goodwin | Villanova University/SAS | 0.64 | 2:21.12 |
| 125Heat — | Caitlin Brooks | Gator Swim Club | 0.58 | 2:21.24 |
| 126Heat — | Camey Rabold | Westerville Aquatic Club/OSU | 0.66 | 2:21.49 |
| 127Heat — | Abby Richter | Boulder City Henderson Swim Te | 0.58 | 2:21.57 |
| 128Heat — | Hannah Lindsey | All Star Aquatics | 0.68 | 2:21.83 |
| 129Heat — | Morgan Tankersley | Greater Tampa Swimming Associa | 0.62 | 2:21.87 |
| 130Heat — | Elise Haan | T2 Aquatics | 0.70 | 2:22.10 |
| 131Heat — | Meghan Lavelle | University Of Iowa | 0.69 | 2:22.39 |
| 132Heat — | Hannah Whiteley | Mason Manta Rays | 0.63 | 2:23.74 |
| 133Heat — | Maria Sheridan | Duke University/MAC | 0.56 | 2:25.15 |
| —Heat — | Tara Halsted | Davis Arden Racing Team | — | |
| —Heat — | Rachel Zilinskas | Athens Bulldog Swim Club/UGA | — | |
| —Heat — | Hannah Moore | NC State University | — | |
| —Heat — | Gabby Deloof | Univ. Of Michigan Swim Team/PA | — | NS |