| 1Heat — | Olivia Smoliga | Athens Bulldog Swim Club/UGA | 0.65 | 59.65 |
| 2Heat — | Amy Bilquist | California Aquatics/CSC | 0.67 | 59.67 |
| 3Heat — | Hannah Stevens | U. of Missouri/GCST | 0.60 | 59.72 |
| 4Heat — | Ali Deloof | Univ. Of Michigan Swim Team/PA | 0.64 | 1:00.09 |
| 5Heat — | Missy Franklin | Colorado Stars/Cal | 0.64 | 1:00.35 |
| 6Heat — | Clara Smiddy | Univ. Of Michigan Swim Team/AKS | 0.64 | 1:00.38 |
| 7Heat — | Natalie Coughlin | California Aquatics | 0.65 | 1:00.75 |
| 8Heat — | Grace Ariola | Waves Bloomington/Normal Y S.T | 0.56 | 1:00.79 |
| 8Heat — | Kathleen Baker | SwimMAC Carolina/UCB | 0.65 | 1:00.79 |
| 10Heat — | Lucie Nordmann | Magnolia Aquatic Club | 0.65 | 1:00.80 |
| 11Heat — | Tasija Karosas | Longhorn Aquatics/UT | 0.63 | 1:01.06 |
| 12Heat — | Danielle Galyer | Kentucky Aquatics/YSSC | 0.52 | 1:01.07 |
| 13Heat — | Regan Smith | Riptide | 0.57 | 1:01.17 |
| 14Heat — | Alex Walsh | Nashville Aquatic Club | 0.67 | 1:01.20 |
| 15Heat — | Annie Ochitwa | Tucson Ford Dealers Aquatics/DSA | 0.69 | 1:01.25 |
| 16Heat — | Taylor Garcia | Tucson Ford Dealers Aquatics/BC | 0.60 | 1:01.32 |
| 17Heat — | Ally Howe | Stanford Swimming/PASA | 0.70 | 1:01.33 |
| 18Heat — | Rachel Bootsma | California Aquatics/AQJT | 0.54 | 1:01.34 |
| 19Heat — | Bridgette Alexander | Kentucky Aquatics | 0.66 | 1:01.35 |
| 20Heat — | Linnea Mack | UCLA Swimming/OAQ | 0.66 | 1:01.52 |
| 21Heat — | Hellen Moffitt | North Carolina Aquatic Club | 0.61 | 1:01.68 |
| 22Heat — | Melanie Klaren | California Aquatics | 0.60 | 1:01.69 |
| 23Heat — | Elizabeth Pelton | California Aquatics/T2 | 0.65 | 1:01.73 |
| 24Heat — | Megan Moroney | Cavalier Swimming/SAS | 0.62 | 1:01.80 |
| 25Heat — | Madison White | UCLA Swimming | 0.64 | 1:01.84 |
| 26Heat — | Caroline Baldwin | North Carolina Aquatic Club | 0.54 | 1:01.86 |
| 27Heat — | Asia Seidt | Lakeside Swim Team | 0.67 | 1:01.89 |
| 28Heat — | Janet Hu | Stanford Swimming/NCAP | 0.52 | 1:01.93 |
| 28Heat — | Kaitlin Harty | YMCA of the North Shore | 0.59 | 1:01.93 |
| 30Heat — | Hannah Weiss | Trojan Swim Club/USC | 0.58 | 1:01.94 |
| 31Heat — | Hanni Leach | Trojan Swim Club/USC | 0.65 | 1:01.95 |
| 32Heat — | Erin Voss | Bluefish Swim Club/WAC | 0.66 | 1:02.03 |
| 33Heat — | Gabby Deloof | Univ. Of Michigan Swim Team/PA | 0.69 | 1:02.04 |
| 34Heat — | Katrina Konopka | Tucson Ford Dealers Aquatics/YSSC | 0.63 | 1:02.09 |
| 35Heat — | Courtney Caldwell | NC State University | 0.58 | 1:02.11 |
| 36Heat — | Katharine Berkoff | Missoula Aquatic Club | 0.67 | 1:02.18 |
| 37Heat — | Cameron McHugh | Tucson Ford Dealers Aquatics/CFSC | 0.61 | 1:02.28 |
| 38Heat — | Candace Cooper | Wolfpack Elite | 0.60 | 1:02.32 |
| 39Heat — | Anika Apostalon | Trojan Swim Club/USC | 0.64 | 1:02.36 |
| 40Heat — | Erin Oeltjen | Club Huskers/MAC | 0.62 | 1:02.37 |
| 41Heat — | Keaton Blovad | Tualatin Hills Swim Club | 0.62 | 1:02.42 |
| 42Heat — | Sarah Hitchens | North Carolina Aquatic Club | 0.67 | 1:02.43 |
| 42Heat — | Brienne Ryan | New York Athletic Club | 0.61 | 1:02.43 |
| 44Heat — | Taylor Schick | Tucson Ford Dealers Aquatics/SA | 0.58 | 1:02.44 |
| 45Heat — | Autumn Haebig | Ozaukee Aquatics | 0.70 | 1:02.45 |
| 46Heat — | Claire Adams | Carmel Swim Club | 0.68 | 1:02.46 |
| 47Heat — | Laura Norman | Texas A & M University/NTN | 0.60 | 1:02.49 |
| 48Heat — | Holly Harper | H2okie Aquatics/GATR | 0.64 | 1:02.52 |
| 48Heat — | Lisa Bratton | Texas A & M University/TCCC | 0.71 | 1:02.52 |
| 50Heat — | Eva Merrell | Aquazot Swim Club | 0.70 | 1:02.53 |
| 51Heat — | Stephanie Nelson | SwimMAC Carolina | 0.61 | 1:02.60 |
| 52Heat — | Melissa Postoll | Northwestern University ST/RAYS | 0.61 | 1:02.65 |
| 53Heat — | Julia Menkhaus | SwimMAC Carolina | 0.67 | 1:02.67 |
| 53Heat — | Claire Brandt | Texas A & M University | 0.63 | 1:02.67 |
| 55Heat — | Micah Bohon | Tennessee Aquatics/UT | 0.58 | 1:02.68 |
| 55Heat — | Callie Dickinson | Tide Swimming | 0.60 | 1:02.68 |
| 57Heat — | Madison Myers | Firestone Akron Swim Team/AKRON | 0.55 | 1:02.76 |
| 58Heat — | Samantha Wicks | Boise YMCA Swim Team | 0.62 | 1:02.78 |
| 59Heat — | Caroline Hauder | SwimMAC Carolina | 0.62 | 1:02.80 |
| 60Heat — | Kyra Sarazen | Irish Aquatics/ND | 0.61 | 1:02.81 |
| 61Heat — | Rhyan White | Wasatch Front Fish Market | 0.72 | 1:02.82 |
| 62Heat — | Emma Schanz | UCLA Swimming/SWAT | 0.68 | 1:02.90 |
| 63Heat — | Maddie Hess | Duke University/BW | 0.61 | 1:02.94 |
| 64Heat — | Alice Treuth | Irish Aquatics/ND | 0.68 | 1:02.96 |
| 65Heat — | Bonnie Brandon | Tucson Ford Dealers Aquatics/MACS | 0.70 | 1:03.00 |
| 66Heat — | Olivia Bray | Virginia Gators | 0.67 | 1:03.06 |
| 67Heat — | Kobie Melton | Huntsville Swim Association | 0.66 | 1:03.09 |
| 68Heat — | Solie Laughlin | Buenaventura Swim Club/UCD | 0.71 | 1:03.12 |
| 69Heat — | Tevyn Waddell | Mitchell Aquatic Club | 0.70 | 1:03.16 |
| 70Heat — | Emma Seiberlich | Phoenixville YMCA | 0.79 | 1:03.19 |
| 71Heat — | Mara Newman | Lake Oswego Swim Club | 0.66 | 1:03.27 |
| 72Heat — | Erin Earley | Hopkins Mariner Swim Team | 0.76 | 1:03.28 |
| 73Heat — | Julia Cook | Aggie Swim Club | 0.76 | 1:03.30 |
| 74Heat — | Lauren Hines | Spider Swimming | 0.70 | 1:03.33 |
| 75Heat — | Elise Haan | T2 Aquatics | 0.68 | 1:03.36 |
| 76Heat — | Catherine Mulquin | Irish Aquatics/ND | 0.63 | 1:03.37 |
| 76Heat — | Kiley Beall | Rice Aquatics/CHES | 0.53 | 1:03.37 |
| 78Heat — | Emily Meilus | Machine Aquatics | 0.57 | 1:03.40 |
| 79Heat — | Ali Galyer | Y-Spartaquatics Swim Club | 0.59 | 1:03.43 |
| 80Heat — | Tristen Ulett | Dynamo Swim Club | 0.66 | 1:03.48 |
| 80Heat — | Catie Deloof | Univ. Of Michigan Swim Team/PA | 0.62 | 1:03.48 |
| 82Heat — | Alex Sumner | Suburban Seahawks Club | 0.61 | 1:03.54 |
| 83Heat — | Phoebe Bacon | Nation's Capital Swim Club | 0.67 | 1:03.56 |
| 84Heat — | Destiny Nelson | Texas Ford Aquatics/Yale | 0.68 | 1:03.57 |
| 85Heat — | Sammie Burchill | Carmel Swim Club | 0.53 | 1:03.58 |
| 85Heat — | Haley Hynes | Kansas City Blazers | 0.68 | 1:03.58 |
| 87Heat — | Aly Tetzloff | Auburn University Swim Club/CPSC | 0.62 | 1:03.59 |
| 87Heat — | Krystal Lara | Asphalt Green Unified Aquatics | 0.57 | 1:03.59 |
| 89Heat — | Jen Marrkand | Cavalier Swimming | 0.59 | 1:03.62 |
| 90Heat — | Rebecca Baxley | Neptune Swimming/UT | 0.63 | 1:03.65 |
| 91Heat — | Riley Lexvold | Aquazot Swim Club | 0.67 | 1:03.68 |
| 92Heat — | Sydney Sell | Lakeside Swim Team/FLOR | 0.64 | 1:03.70 |
| 93Heat — | Mickayla Hinkle | Duke University/SCSC | 0.63 | 1:03.75 |
| 94Heat — | Blair Wegescheide | University of Pittsburgh | 0.79 | 1:03.78 |
| 95Heat — | Evie Pfeifer | CSP Tideriders | 0.71 | 1:03.79 |
| 96Heat — | Ashlyn Schoof | Schroeder YMCA Swim Team | 0.60 | 1:03.80 |
| 97Heat — | Katherine Parker | SwimAtlanta | 0.69 | 1:03.81 |
| 98Heat — | Lillie Hosack | Ozaukee Aquatics | 0.71 | 1:03.82 |
| 99Heat — | Amanda Smith | Northern KY Clippers Swimming | 0.56 | 1:03.83 |
| 100Heat — | Victoria Edwards | Longhorn Aquatics | 0.61 | 1:03.84 |
| 101Heat — | Kenzie Buss | University of Louisville | 0.69 | 1:03.85 |
| 102Heat — | Caitlin Brooks | Gator Swim Club | 0.59 | 1:03.89 |
| 103Heat — | Tonner Debeer | Triton Swimming | 0.58 | 1:03.91 |
| 104Heat — | Hannah Gouger | Loyola Blakefield Aquatics | 0.67 | 1:03.94 |
| 105Heat — | Makenna Wilson | Louisiana State University | 0.60 | 1:03.95 |
| 106Heat — | Alexis Mitcheltree | Eastern Michigan University/ASC | 0.62 | 1:03.98 |
| 107Heat — | Christina Leander | Hurricane Aquatics | 0.72 | 1:03.99 |
| 108Heat — | Abby Richter | Boulder City Henderson Swim Te | 0.58 | 1:04.00 |
| 109Heat — | Meghan Lavelle | University Of Iowa | 0.66 | 1:04.01 |
| 110Heat — | Bayley Stewart | Fort Collins Area Swim Team | 0.65 | 1:04.08 |
| 110Heat — | Marie Chamberlain | Indiana University/CCYM | 0.59 | 1:04.08 |
| 112Heat — | Bailey Grinter | Edwardsville YMCA | 0.76 | 1:04.10 |
| 112Heat — | Matti Harrison | Phoenix Swim Club | 0.78 | 1:04.10 |
| 114Heat — | Lacey Locke | Northwestern University ST/CSC | 0.58 | 1:04.11 |
| 115Heat — | Becca Postoll | Univ. Of Michigan Swim Team/MAC | 0.60 | 1:04.13 |
| 116Heat — | Emily Slabe | North Carolina Aquatic Club | 0.67 | 1:04.14 |
| 116Heat — | Kendall Shields | Austin Swim Club | 0.64 | 1:04.14 |
| 116Heat — | Anu Nihipali | Unattached | 0.64 | 1:04.14 |
| 119Heat — | Sara Metzsch | Tualatin Hills Swim Club | 0.67 | 1:04.15 |
| 120Heat — | Alyssa Marsh | SwimMAC Carolina | 0.64 | 1:04.18 |
| 120Heat — | Morgan McCafferty | Club Huskers/GCST | 0.59 | 1:04.18 |
| 122Heat — | Colleen Humel | San Jose State Spartans | 0.68 | 1:04.19 |
| 123Heat — | Caitlin Cooper | Cavalier Swimming | 0.61 | 1:04.22 |
| 124Heat — | Morgan McCormick | Unattached | 0.67 | 1:04.24 |
| 124Heat — | Marcie Maguire | Ridgefield Aquatic Club | 0.66 | 1:04.24 |
| 126Heat — | Maddie Wallis | Gator Swim Club | 0.63 | 1:04.27 |
| 127Heat — | Katie Kelsoe | University Of Alabama Swim Clu | 0.60 | 1:04.28 |
| 128Heat — | Carly Quast | Aquajets Swim Team | 0.59 | 1:04.30 |
| 128Heat — | Abby Kochevar | University of Denver Hilltoppe | 0.62 | 1:04.30 |
| 130Heat — | Izzy Herb | St Charles Swim Team | 0.70 | 1:04.32 |
| 131Heat — | Sophie Krivokapic-Zhou | United States | 0.72 | 1:04.41 |
| 132Heat — | Lainey Visscher | Oregon City Swim Team | 0.59 | 1:04.46 |
| 133Heat — | Jamie Kolar | NASA Wildcat Aquatics | 0.68 | 1:04.47 |
| 134Heat — | Aria Bernal | Katy Aquatic Team For Youth | 0.62 | 1:04.53 |
| 135Heat — | Camey Rabold | Westerville Aquatic Club/OSU | 0.61 | 1:04.59 |
| 136Heat — | Kacey Oberlander | York YMCA | 0.75 | 1:04.64 |
| 137Heat — | Mary Cayten Brakefield | Nashville Aquatic Club | 0.57 | 1:04.67 |
| 138Heat — | Ashley Pollok | The Woodlands Swim Team | 0.66 | 1:04.72 |
| 138Heat — | Grace Haskett | Indiana Swim Team | 0.55 | 1:04.72 |
| 138Heat — | Camryn Forbes | Butler Y Aquatic Club | 0.57 | 1:04.72 |
| 141Heat — | Christie Chong | Pleasanton Seahawks | 0.61 | 1:04.81 |
| 142Heat — | Beata Nelson | Madison Aquatic Club | 0.78 | 1:04.82 |
| 143Heat — | Courtney Mykkanen | Irvine Novaquatics | 0.69 | 1:04.83 |
| 144Heat — | Ashlyn Koletic | Wolfpack Elite/NCS | 0.58 | 1:04.90 |
| 145Heat — | Heidi Vanderwel | King Aquatic Club/Yale | 0.65 | 1:04.91 |
| 146Heat — | Allie Szekely | Central Bucks Swim Team | 0.63 | 1:04.92 |
| 147Heat — | Joanna Wu | Scarlet Aquatics | 0.55 | 1:04.97 |
| 148Heat — | Zoe Cosgrove | Davis Arden Racing Team | 0.66 | 1:05.06 |
| 149Heat — | Christina Paspalas | Phoenix Aquatic Club | 0.62 | 1:05.11 |
| 150Heat — | Emily Eastin | SOCAL Aquatics Association | 0.60 | 1:05.14 |
| 151Heat — | Natalie Mudd | Southeastern Swim Club | 0.65 | 1:05.32 |
| 152Heat — | Sam Siebenaller | Menomonee Falls Swim Club | 0.64 | 1:05.47 |
| 153Heat — | Hannah Whiteley | Mason Manta Rays | 0.65 | 1:05.51 |
| 154Heat — | Zoe Avestruz | Aquajets Swim Team/MINN | 0.64 | 1:05.75 |
| 155Heat — | Remedy Rule | Longhorn Aquatics/UT | 0.63 | 1:05.81 |
| —Heat — | Lisa Boyce | Longhorn Aquatics/PUST | 0.67 | DQ |
| —Heat — | Ashley Leclair | University of Louisville | 0.59 | DQ |