| 1Heat — | Madison Kennedy | SwimMAC Carolina | 0.64 | 24.52 |
| 2Heat — | Simone Manuel | Stanford Swimming/FCST | 0.68 | 24.57 |
| 3Heat — | Abbey Weitzeil | Canyons Aquatic Club | 0.68 | 24.58 |
| 4Heat — | Olivia Smoliga | Athens Bulldog Swim Club/UGA | 0.70 | 24.78 |
| 5Heat — | Katrina Konopka | Tucson Ford Dealers Aquatics/YSSC | 0.71 | 24.90 |
| 6Heat — | Dana Vollmer | California Aquatics | 0.71 | 24.96 |
| 7Heat — | Ivy Martin | Wisconsin Aquatics | 0.66 | 25.05 |
| 8Heat — | Kelsi Worrell | University of Louisville | 0.63 | 25.12 |
| 9Heat — | Amanda Weir | SwimAtlanta | 0.74 | 25.15 |
| 10Heat — | Lia Neal | Stanford Swimming/AGUA | 0.74 | 25.16 |
| 11Heat — | Maddie Locus | SwimAtlanta | 0.67 | 25.27 |
| 12Heat — | Katie Meili | SwimMAC Carolina/NYAC | 0.64 | 25.37 |
| 13Heat — | Anika Apostalon | Trojan Swim Club/USC | 0.66 | 25.43 |
| 14Heat — | Taylor Schick | Tucson Ford Dealers Aquatics/SA | 0.64 | 25.48 |
| 15Heat — | Caitlin Cooper | Cavalier Swimming | 0.68 | 25.51 |
| 16Heat — | Anya Goeders | Mako Swim Team | 0.67 | 25.53 |
| 17Heat — | Margo Geer | Tucson Ford Dealers Aquatics | 0.69 | 25.57 |
| 18Heat — | Chase Kinney | Wisconsin Aquatics/NCY | 0.72 | 25.58 |
| 19Heat — | Marta Ciesla | Pine Crest Swimming | 0.71 | 25.59 |
| 20Heat — | Kendyl Stewart | North Coast Aquatics/USC | 0.74 | 25.59 |
| 21Heat — | Casey Fanz | Poseidon Swimming Inc | 0.64 | 25.60 |
| 21Heat — | Daria Pyshnenko | Cats Aquatic Team | 0.71 | 25.60 |
| 23Heat — | Maddie Murphy | Crow Canyon Country Club Shark | 0.72 | 25.66 |
| 24Heat — | Jessica Hardy | Golden West Swim Club | 0.69 | 25.68 |
| 25Heat — | Harper Bruens | University Of Tennessee/BRST | 0.73 | 25.70 |
| 26Heat — | Caroline Baldwin | North Carolina Aquatic Club | 0.69 | 25.71 |
| 27Heat — | Kaitlyn Johnson | Club Mountaineer Aquatics | 0.63 | 25.75 |
| 28Heat — | Rebecca Millard | Irvine Novaquatics/UT | 0.73 | 25.76 |
| 28Heat — | Faith Johnson | University Of Tennessee | 0.68 | 25.76 |
| 30Heat — | Anna Patterson | U. of Missouri/CFSC | 0.68 | 25.79 |
| 31Heat — | Maggie Gruber | H2okie Aquatics/VTECH | 0.65 | 25.83 |
| 32Heat — | Kate Douglass | Westchester Aquatic Club | 0.71 | 25.84 |
| 33Heat — | Isabel Ivey | Gator Swim Club | 0.75 | 25.86 |
| 34Heat — | Eva Merrell | Aquazot Swim Club | 0.70 | 25.88 |
| 34Heat — | Mallory Comerford | University of Louisville/SWYM | 0.74 | 25.88 |
| 36Heat — | Lauren Pitzer | Lakeside Aquatic Club | 0.75 | 25.91 |
| 36Heat — | Felicia Lee | Stanford Swimming/NBAC | 0.66 | 25.91 |
| 38Heat — | Dani Barbiea | U. of Missouri/RAYS | 0.66 | 25.93 |
| 39Heat — | Ali Deloof | Univ. Of Michigan Swim Team/PA | 0.72 | 25.94 |
| 40Heat — | Haylee Knight | Louisiana State University | 0.68 | 25.95 |
| 41Heat — | Veronica Burchill | Carmel Swim Club | 0.67 | 25.96 |
| 42Heat — | Janet Hu | Stanford Swimming/NCAP | 0.59 | 25.97 |
| 42Heat — | Courtney Caldwell | NC State University | 0.69 | 25.97 |
| 44Heat — | Krista Duffield | Wolfpack Elite/NCS | 0.73 | 25.98 |
| 44Heat — | Marissa Berg | Wisconsin Aquatics | 0.65 | 25.98 |
| 46Heat — | Megan Burns | University at Buffalo Swim Tea | 0.69 | 26.01 |
| 46Heat — | Dina Rommel | Cavalier Swimming/TTSC | 0.69 | 26.01 |
| 46Heat — | Natalie Labonge | NC State University | 0.68 | 26.01 |
| 49Heat — | Allie Wooden | Trojan Swim Club/CPM | 0.69 | 26.04 |
| 49Heat — | Amalie Fackenthal | Davis Arden Racing Team | 0.60 | 26.04 |
| 51Heat — | Amanda Kendall | Mason Makos Swim Team | 0.69 | 26.05 |
| 52Heat — | Morgan Tankersley | Greater Tampa Swimming Associa | 0.67 | 26.06 |
| 53Heat — | Alyssa Marsh | SwimMAC Carolina | 0.68 | 26.07 |
| 53Heat — | Olivia Calegan | Greater Nebraska Swim Team | 0.75 | 26.07 |
| 55Heat — | Natalie Hinds | City of Midland Swim Team/FLOR | 0.63 | 26.08 |
| 55Heat — | Rochelle Dong | Rancho San Dieguito/PENN | 0.60 | 26.08 |
| 55Heat — | Jamie Stone | Mount Hood Aquatics | 0.73 | 26.08 |
| 58Heat — | Aly Tetzloff | Auburn University Swim Club/CPSC | 0.67 | 26.10 |
| 58Heat — | Kristen Vredeveld | California Aquatics | 0.70 | 26.10 |
| 60Heat — | Kristin Malone | Texas A & M University/SSC | 0.70 | 26.11 |
| 61Heat — | Bailey Scott | University Of Alabama Swim Clu | 0.67 | 26.12 |
| 62Heat — | Ashton Ellzey | City of Mobile Swim Associatio/AUB | 0.65 | 26.13 |
| 63Heat — | Chelsea Bailey | King Aquatic Club | 0.70 | 26.15 |
| 63Heat — | Annie Ochitwa | Tucson Ford Dealers Aquatics/DSA | 0.70 | 26.15 |
| 65Heat — | Morgan Hill | Nation's Capital Swim Club | 0.72 | 26.16 |
| 66Heat — | Melissa Gates | Trojan Swim Club | 0.71 | 26.17 |
| 66Heat — | Rebecca Weiland | Minnetonka Swim Club | 0.69 | 26.17 |
| 68Heat — | Avery Braunecker | Academy Bullets Swim Club | 0.69 | 26.18 |
| 69Heat — | Katie Saloky | Penn State University | 0.61 | 26.19 |
| 70Heat — | Elise Garcia | SOCAL Aquatics Association | 0.64 | 26.20 |
| 70Heat — | Brooke Bauer | Lakeside Swim Team | 0.70 | 26.20 |
| 70Heat — | Grace Ariola | Waves Bloomington/Normal Y S.T | 0.69 | 26.20 |
| 70Heat — | Taylor Wilcox | Unattached | 0.73 | 26.20 |
| 70Heat — | Claire Brandt | Texas A & M University | 0.64 | 26.20 |
| 75Heat — | Megan Moroney | Cavalier Swimming/SAS | 0.64 | 26.21 |
| 75Heat — | Macie McNichols | Ohio State University | 0.63 | 26.21 |
| 75Heat — | Lauren Green | Quicksilver Swimming | 0.63 | 26.21 |
| 75Heat — | Grace Countie | Marlins of Raleigh | 0.78 | 26.21 |
| 79Heat — | Linnea Mack | UCLA Swimming/OAQ | 0.74 | 26.22 |
| 79Heat — | Carly Mercer | Washington Township Swim Club | 0.65 | 26.22 |
| 79Heat — | Danielle Nack | Mantas Swim Club/MINN | 0.75 | 26.22 |
| 82Heat — | Taryn Collura | Club Huskers | 0.68 | 26.23 |
| 82Heat — | Ky-lee Perry | Wilton Y Wahoos Swim Club | 0.69 | 26.23 |
| 82Heat — | Nika Blank | Peddie Aquatic Association | 0.64 | 26.23 |
| 85Heat — | Catie Deloof | Univ. Of Michigan Swim Team/PA | 0.70 | 26.24 |
| 86Heat — | Mimi Schneider | Academy Bullets Swim Club/UT | 0.68 | 26.25 |
| 86Heat — | Raena Eldridge | Texas A & M University/RACE | 0.64 | 26.25 |
| 86Heat — | Bethany Galat | Texas A & M University/SBSC | 0.61 | 26.25 |
| 89Heat — | Alex Cleveland | Tennessee Aquatics/ZSC | 0.71 | 26.26 |
| 90Heat — | Anna-julia Kutsch | JCC-Aquatic Club | 0.74 | 26.29 |
| 91Heat — | Eryn Eddy | Cavalier Swimming/LOVE | 0.72 | 26.30 |
| 91Heat — | Erika Brown | SwimMAC Carolina | 0.73 | 26.30 |
| 93Heat — | Kayla Filipek | Academy Bullets Swim Club | 0.70 | 26.31 |
| 93Heat — | Emily Murphy | Team Pittsburgh Aquatics/PITT | 0.64 | 26.31 |
| 93Heat — | Kenisha Liu | Brea Aquatics | 0.62 | 26.31 |
| 93Heat — | Catherine Mulquin | Irish Aquatics/ND | 0.68 | 26.31 |
| 97Heat — | Delaney Barnard | Indiana University/ATAC | 0.69 | 26.32 |
| 98Heat — | Victoria Trost | U. of Missouri/CIA | 0.70 | 26.33 |
| 99Heat — | Ingrid Wall | New Trier Swim Club | 0.71 | 26.34 |
| 100Heat — | Anelise Diener | Streamline Aquatics/UT | 0.73 | 26.37 |
| 101Heat — | Mandie Siehs | Unattached OZ | 0.71 | 26.38 |
| 101Heat — | Kaitlyn Dressel | Area Tallahassee Aquatic Club | 0.69 | 26.38 |
| 103Heat — | Trude Rothrock | Carmel Swim Club | 0.67 | 26.40 |
| 104Heat — | Miki Dahlke | North Bay Aquatics | 0.67 | 26.41 |
| 104Heat — | Annie Miller | Tribe Aquatics/DADS | 0.76 | 26.41 |
| 106Heat — | Abby Jagdfeld | Unattached WA/WEST | 0.73 | 26.43 |
| 106Heat — | Katie Smith | PACK Swim Team of Pittsford | 0.65 | 26.43 |
| 106Heat — | Lisa Boyce | Longhorn Aquatics/PUST | 0.72 | 26.43 |
| 106Heat — | Corrin Van Lanen | Ohio University | 0.63 | 26.43 |
| 110Heat — | Katherine Wagner | Canyons Aquatic Club | 0.71 | 26.44 |
| 111Heat — | Rachael Acker | California Aquatics | 0.72 | 26.46 |
| 111Heat — | Hannah Raspopovich | Firestone Akron Swim Team/AKRON | 0.65 | 26.46 |
| 113Heat — | Kasey Schmidt | Cavalier Swimming/BSS | 0.66 | 26.47 |
| 113Heat — | Mary Warren | Northwestern University ST/LAKE | 0.67 | 26.47 |
| 115Heat — | Ashley Sutherland | Tucson Ford Dealers Aquatics/KING | 0.66 | 26.48 |
| 115Heat — | Caroline Simmons | Performance Aquatics | 0.74 | 26.48 |
| 115Heat — | Nora Fullenkamp | Dayton Raiders | 0.71 | 26.48 |
| 118Heat — | Katie Schorr | Southwest Stars Swim Club | 0.72 | 26.49 |
| 119Heat — | Hellen Moffitt | North Carolina Aquatic Club | 0.76 | 26.51 |
| 119Heat — | Brittany Aoyama | Tiger Aquatics/BSU | 0.61 | 26.51 |
| 121Heat — | Adriana Grabski | H2okie Aquatics/VTECH | 0.69 | 26.53 |
| 121Heat — | Victoria Griffin | Falcon Aquatics | 0.64 | 26.53 |
| 123Heat — | Meredith Vay | Gamecock Aquatics/SCAR | 0.62 | 26.54 |
| 123Heat — | Miranda Donley | Ohio State Swim Club | 0.65 | 26.54 |
| 125Heat — | Gretchen Walsh | Nashville Aquatic Club | 0.65 | 26.55 |
| 125Heat — | Cheyenne Meek | Ohio State University | 0.65 | 26.55 |
| 125Heat — | Rachel Hayden | U. of Missouri/CSC | 0.72 | 26.55 |
| 128Heat — | Taylor Johnson | San Diego State | 0.56 | 26.56 |
| 128Heat — | Olivia Schmelzer | Lancaster YMCA Swim Team | 0.75 | 26.56 |
| 128Heat — | Kimmie Kreuzberger | Gaucho Aquatics/MP | 0.68 | 26.56 |
| 128Heat — | Sammie Bosma | Stingrays | 0.78 | 26.56 |
| 128Heat — | Jaimie Miller | Tribe Aquatics/DADS | 0.77 | 26.56 |
| 133Heat — | Bailey Grinter | Edwardsville YMCA | 0.74 | 26.58 |
| 133Heat — | Laine Reed | Cavalier Swimming | 0.72 | 26.58 |
| 135Heat — | Katie Kicklighter | YMCA of Central Florida | 0.79 | 26.60 |
| 136Heat — | Morgan Wice-Roslin | Unattached/UDen | 0.73 | 26.62 |
| 137Heat — | Gillian St. John | Swim Utah | 0.72 | 26.63 |
| 137Heat — | Lexie Lupton | Texas A & M University/Eagle | 0.66 | 26.63 |
| 139Heat — | Andrea Hillen | Enfinity Aquatic Club/WOLF | 0.70 | 26.64 |
| 140Heat — | Kathleen Baker | SwimMAC Carolina/UCB | 0.70 | 26.66 |
| 140Heat — | Alex Meyers | Stanford Swimming/SSTY | 0.70 | 26.66 |
| 142Heat — | Alayna Connor | The Dolphins Portland Swimming | 0.68 | 26.68 |
| 142Heat — | Kelley Conrad | Scottsdale Aquatic Club | 0.73 | 26.68 |
| 142Heat — | Brooke Stenstrom | Denver Swim Academy | 0.63 | 26.68 |
| 145Heat — | Annie Jongekrijg | Ohio State University | 0.72 | 26.70 |
| 145Heat — | Kyla Valls | Miami Swimming/R.E. | 0.67 | 26.70 |
| 145Heat — | Marie Ballenger | The Olympic Club | 0.68 | 26.70 |
| 148Heat — | Stanzi Moseley | Canyons Aquatic Club | 0.70 | 26.72 |
| 148Heat — | Maddie Rusch | Duke University/NCY | 0.72 | 26.72 |
| 148Heat — | Leah Goldman | Palo Alto Stanford Aquatics | 0.66 | 26.72 |
| 151Heat — | Kenzie Margroum | Navy Swimming/CLPR | 0.74 | 26.73 |
| 152Heat — | Morgan Ginnis | King Aquatic Club | 0.71 | 26.74 |
| 152Heat — | Ragen Engel | Firestone Akron Swim Team/AKRON | 0.67 | 26.74 |
| 154Heat — | Nora McCullagh | SwimMAC Carolina/UT | 0.73 | 26.75 |
| 154Heat — | Beata Nelson | Madison Aquatic Club | 0.75 | 26.75 |
| 156Heat — | Emily Zimcosky | Club Mountaineer Aquatics | 0.74 | 26.76 |
| 156Heat — | Leah Hatayama | Fresno State Swimming | 0.61 | 26.76 |
| 158Heat — | Gabby Dang | Wave Aquatics | 0.69 | 26.77 |
| 159Heat — | Kate Nezelek | Poseidon Swimming Inc | 0.73 | 26.78 |
| 160Heat — | Rachael Dzierzak | Ohio State University/SYL | 0.78 | 26.83 |
| 160Heat — | Maddie Hess | Duke University/BW | 0.71 | 26.83 |
| 162Heat — | Thuymi Dinh | Upper Main Line YMCA/NAVY | 0.66 | 26.84 |
| 162Heat — | Lauren Vosseler | Gaucho Aquatics/GWSC | 0.75 | 26.84 |
| 164Heat — | Ashley Volpenhein | Mason Manta Rays | 0.68 | 26.85 |
| 164Heat — | Kaitlyn Ruffing | Unattached | 0.76 | 26.85 |
| 164Heat — | Katie Cronin | Gator Swim Club | 0.71 | 26.85 |
| 167Heat — | Kaitlin Jones | Penn State University/QUEST | 0.73 | 26.86 |
| 168Heat — | Hannah Zurmuhl | Central Bucks Swim Team | 0.70 | 26.97 |
| 169Heat — | Lauren Heller | Lake Erie Silver Dolphins | 0.72 | 27.00 |
| 169Heat — | Alex Bilunas | Club Huskers/GOAL | 0.69 | 27.00 |
| 171Heat — | Grace Zhao | Palo Alto Stanford Aquatics | 0.70 | 27.01 |
| 172Heat — | Ashlyn Koletic | Wolfpack Elite/NCS | 0.68 | 27.07 |
| 173Heat — | Emily Allen | Tennessee Aquatics/UT | 0.74 | 27.08 |
| 174Heat — | Evan Swenson | Unattached | 0.72 | 27.28 |
| 175Heat — | Kylie Vogel | Boilermaker Aquatics/PUR | 0.70 | 27.58 |
| 176Heat — | Syd Fisher | George Mason University | 0.78 | 27.69 |
| 177Heat — | Sophie Skinner | Northern KY Clippers Swimming | 0.76 | 27.78 |
| 178Heat — | Zoe Huddleston | University of Denver Hilltoppe/DU | 0.71 | 27.80 |
| —Heat — | Isabelle Henig | Palo Alto Stanford Aquatics | 0.62 | DQ |
| —Heat — | Zoe Avestruz | Aquajets Swim Team/MINN | — | NS |