| 1Heat — | Collin Holgerson | SwimAtlanta | — | 52.57 |
| 2Heat — | Jake Lloyd | Central Ohio Aqu | — | 53.26 |
| 3Heat — | Ian Heysen | Blue Dolfins | — | 53.34 |
| 4Heat — | Austin Carpenter | Central Ohio Aqu | — | 53.43 |
| 5Heat — | Matthew Wolfle | NOVA of Virginia | — | 53.46 |
| 6Heat — | Lucas Ackermann | Franklin Regiona | — | 53.47 |
| 7Heat — | Prakhanthi Rai | Bolles School Sh | — | 53.51 |
| 8Heat — | Jude Banks | Blazing Barracud | — | 53.86 |
| 9Heat — | Ian Disosway | Fast Falcons | — | 53.91 |
| 10Heat — | Ian Call | Memphis Thunder- 1:00.49L | — | 53.92 |
| 11Heat — | Ryan McDonald | Badger Swim Club | — | 54.07 |
| 12Heat — | Kyle Li | Dragon Swim Team | — | 54.14 |
| 13Heat — | Lucas Young | Bolles School Sh | — | 54.20 |
| 14Heat — | Reef McMeeking | Laker Swim | — | 54.30 |
| 15Heat — | Luke Zardavets | Bolles School Sh | — | 54.33 |
| 16Heat — | Wilson York | Lakeside Swim Te | — | 54.36 |
| 17Heat — | Yi Zheng | Carmel Swim Club | — | 54.53 |
| 18Heat — | Zack Kusch | Streamline Aquat | — | 54.56 |
| 19Heat — | Eren Donmez | Old Dominion Aqu | — | 54.60 |
| 20Heat — | Raul Antadillas | Montverde Aquati 1:04.03L | — | 54.73 |
| 21Heat — | Landon Hall | Atomic City Aqua | — | 54.75 |
| 22Heat — | Juan Diego Leon | TS Aquatics-FG 1:04.44L | — | 54.86 |
| 23Heat — | Thor Johannessen | Blazing Barracud 1:04.88L | — | 55.00 |
| 24Heat — | Adam Wang | Bolles School Sh | — | 55.03 |
| 25Heat — | Gerhardt Hoover | Commonwealth Swi | — | 55.05 |
| 26Heat — | Javier Diaz Lopez | Baylor Swim Club 1:05.42L | — | 55.10 |
| 27Heat — | Spencer Kinsey | Bolles School Sh 1:04.80L | — | 55.12 |
| 28Heat — | Louis Joos | Bolles School Sh | — | 55.13 |
| 29Heat — | Matthew Cuadros | Center Grove Aqu 1:04.31L | — | 55.15 |
| 30Heat — | Yehor Maistruk | Jersey Aquatic C | — | 55.18 |
| 31Heat — | Issac Adanin | Club Wolverine-M 1:05.14L | — | 55.24 |
| 32Heat — | Evan Summers | Nittany Lion Aqu | — | 55.32 |
| 33Heat — | Camden Cook | SwimAtlanta-GA 1:03.28L | — | 55.35 |
| 34Heat — | Owen Durham | Lakeside Swim Te 1:04.76L | — | 55.51 |
| 35Heat — | Everett Losch | Lake Erie Silver 1:04.98L | — | 55.52 |
| 36Heat — | Tyler Burton | Enfinity Aquatic 1:05.04L | — | 55.54 |
| 37Heat — | Oliver Hulse | Upper Arlington- 1:03.78L | — | 55.59 |
| 38Heat — | Dominic Impens | YMCA of Upper Pa 1:05.66L | — | 55.60 |
| 39Heat — | Holden Skidmore | Sharks Swim Club | — | 55.66 |
| 40Heat — | Ting Yuk Cobi Wu | Montverde Aquati 1:05.04L | — | 55.68 |
| 41Heat — | Josiah Collins | Fort Wayne Swim- 1:05.17L | — | 55.70 |
| 42Heat — | Joey Rojek | Valparaiso Swim- 1:05.07L | — | 55.73 |
| 43Heat — | Jack Martin | SwimMAC Carolina 1:05.22L | — | 55.80 |
| 44Heat — | Gavin Tran | Hershey Aquatic- | — | 55.85 |
| 45Heat — | Camren Turowski | dROP Aquatics | — | 55.85 |
| 46Heat — | Parker Van Olst | New Wave Swim Te 1:04.77L | — | 55.91 |
| 47Heat — | Christian Guettler | Saint Petersburg 1:05.19L | — | 55.92 |
| 48Heat — | Fin McLane | Commonwealth Swi 1:05.49L | — | 55.96 |
| 49Heat — | Caden Mahl | Central Ohio Aqu | — | 55.98 |
| 50Heat — | Remi Angerer | Auburn Aquatics- 1:04.43L | — | 55.99 |
| 51Heat — | Oliver Kiss | Scarlet Aquatics | — | 56.03 |
| 52Heat — | Sawyer Hackett | Swim Melbourne-F 1:05.08L | — | 56.04 |
| 53Heat — | Matthew Liu | Eagle Swim Team- 1:05.24L | — | 56.12 |
| 54Heat — | Joseph Campagnola | West Florida Lig | — | 56.14 |
| 55Heat — | Austin Klemmer | SwimAtlanta | — | 56.27 |
| 56Heat — | Blake Valyo | Raleigh Swimming 1:05.85L | — | 56.31 |
| 57Heat — | Rinzen Sherpa | Mount Lebanon Aq 1:05.89L | — | 56.33 |
| 58Heat — | Bennett Aldrich | Laker Swim | — | 56.34 |
| 59Heat — | Seth Morgan | Rocky Mount Fami | — | 56.35 |
| 60Heat — | Jared Berke | Saint Andrew's A | — | 56.36 |
| 61Heat — | Tommy Watson | Williamsburg Aqu 1:05.85L | — | 56.38 |
| 62Heat — | Wil Jones | Kentucky Aquatic | — | 56.42 |
| 63Heat — | Peyton Harrison | Lakeside Swim Te | — | 56.46 |
| 64Heat — | Elliott Angkriwan | Bolles School Sh 1:05.86L | — | 56.46 |
| 65Heat — | Will Von Doussa | Metro Area Life- 1:05.46L | — | 56.50 |
| 66Heat — | Brogan Wilson | Cincinnati Marli 1:05.82L | — | 56.55 |
| 67Heat — | Charlie Russell | Bolles School Sh 1:04.53L | — | 56.56 |
| 68Heat — | Sebastian Tith | Upper Main Line- 1:03.68L | — | 56.62 |
| 69Heat — | Tripp Cooler | SwimMAC Carolina 1:05.78L | — | 56.63 |
| 70Heat — | Jack Clippinger | SwimRVA-VA 1:05.49L | — | 56.65 |
| 71Heat — | Liam Clark | Mason Manta Rays 1:05.52L | — | 56.67 |
| 72Heat — | Cullen Cashman | South Shore YMCA 1:04.27L | — | 56.71 |
| 73Heat — | Ethan Tu | Mason Manta Rays 1:05.15L | — | 56.74 |
| 74Heat — | Ben Blomgren | Dynamo Swim Club 1:04.46L | — | 56.75 |
| 75Heat — | Ben Rines | Blue Dolfins | — | 56.80 |
| 76Heat — | Jonathan Vogt | Enfinity Aquatic 1:05.21L | — | 56.86 |
| 77Heat — | Ian Malone | Greater Pensacol | — | 56.87 |
| 78Heat — | Nate Stebbins | Birmingham Blue- | — | 56.90 |
| 79Heat — | Ethan Chipriano | Delaware Swim Te 1:03.36L | — | 56.93 |
| 80Heat — | Caleb Caponera | Florida Atlantic 1:05.85L | — | 57.07 |
| 81Heat — | Danny Saunders | H2okie Aquatics- 1:05.89L | — | 57.07 |
| 82Heat — | Brandon Anand | Scarlet Aquatics 1:05.54L | — | 57.09 |
| 83Heat — | Ivan Blystiv | Concord Swim Clu 1:05.11L | — | 57.14 |
| 84Heat — | Charlie Camplin | Marlins Of Ralei 1:05.51L | — | 57.18 |
| 85Heat — | Christopher Koch | Athens Bulldog S | — | 57.20 |
| 86Heat — | Brayden Hale | Cheshire Y Sea D | — | 57.54 |
| 87Heat — | Trey Chesney | Jupiter Dragons- | — | 57.70 |
| 88Heat — | Alex Aivalotis | Team Pittsburgh- 1:05.64L | — | 57.73 |
| 89Heat — | Charlie McCuiston | Atlantis Swimmin 1:04.59L | — | 57.77 |
| 90Heat — | Parker Barnett | Huntsville Swim- | — | 57.81 |
| 91Heat — | Griffin Barents Froma | Westfield Aquati 1:05.09L | — | 57.82 |
| 92Heat — | Ivan Karpau | Crimson Aquatics 1:05.66L | — | 57.87 |
| 93Heat — | Akay Say | Scarlet Aquatics 1:06.13L | — | 57.89 |
| 94Heat — | Daniel Snitkovsky | Sarasota Tsunami | — | 57.94 |
| 95Heat — | Edward Johnson | NOVA of Virginia 1:04.96L | — | 58.05 |
| 96Heat — | Max Baumgartner | Huntsville Swim- | — | 58.34 |
| 97Heat — | Sam Cavanaugh | Team Pittsburgh- | — | 58.51 |
| 98Heat — | Oliver Cannings | Gulliver Swim Cl 1:07.29L | — | 58.80 |
| 99Heat — | Carter Medlin | Mecklenburg Swim | — | 58.81 |
| —Heat — | Charley Sutorus | SwimMAC Carolina | — | DQ |
| —Heat — | Joe Warnagiris | NOVA of Virginia | — | |
| —Heat — | Josh Wilder | Team Greenville- | — | |
| —Heat — | Owen Ekk | Area Tallahassee | — | |