| 1Heat — | Ian Heysen | Blue Dolfins | 0.71 | 53.52 |
| 2Heat — | Josh Bey | Highland Hurrica | 0.63 | 53.59 |
| 3Heat — | Noah Cakir | Team Suffolk | 0.69 | 53.66 |
| 4Heat — | Gabe Nunziata | Old Dominion Aqu | 0.65 | 53.72 |
| 5Heat — | Giulian Martin | Hurricane Aquati | 0.64 | 53.83 |
| 6Heat — | Jonah Lee | Valparaiso Swim- | 0.60 | 53.87 |
| 7Heat — | Jason Zhao | Mason Manta Rays | 0.60 | 53.93 |
| 8Heat — | Austin Carpente | Central Ohio Aqu | 0.64 | 53.97 |
| 9Heat — | Ian Disosway | Fast Falcons | 0.65 | 53.98 |
| 10Heat — | Luke Zardavets | Planet Swim Aqua | 0.61 | 54.27 |
| 11Heat — | Collin Holgerso | SwimAtlanta | 0.66 | 54.44 |
| 12Heat — | Lucas Young | Bolles School Sh | 0.66 | 54.50 |
| 13Heat — | Wilson York | Lakeside Swim Te | 0.64 | 54.51 |
| 14Heat — | Eldad Zamir | Bolles School Sh | 0.67 | 54.52 |
| 15Heat — | Toby Herzog | Upper Dublin Aqu | 0.65 | 54.64 |
| 16Heat — | George Groves | Commonwealth Swi | 0.66 | 54.71 |
| 17Heat — | Cohen Chiam | Bolles School Sh | 0.65 | 54.74 |
| 18Heat — | Seth Morgan | Rocky Mount Fami | 0.64 | 54.74 |
| 19Heat — | Caiden Bowers | Mercersburg Acad | 0.62 | 54.76 |
| 20Heat — | Jack Mainville | Planet Swim Aqua 1:05.87L | 0.69 | 54.76 |
| 21Heat — | Joseph Campagno | West Florida Lig | 0.65 | 54.81 |
| 22Heat — | Louis Joos | Bolles School Sh | 0.61 | 54.84 |
| 23Heat — | Trent Hollandsw | H2okie Aquatics- | 0.68 | 54.85 |
| 24Heat — | Ryder Hicks | Mason Manta Rays 1:05.00L | 0.74 | 54.97 |
| 25Heat — | Yehor Maistruk | Jersey Aquatic C 1:04.54L | 0.64 | 54.98 |
| 26Heat — | Dane Hogan | Tac Titans | 0.62 | 55.05 |
| 27Heat — | Yi Zheng | Carmel Swim Club 1:05.73L | 0.68 | 55.08 |
| 28Heat — | Braynsly Dirksz | Azura Florida Aq | 0.63 | 55.11 |
| 29Heat — | Walker Bailey | Mason Manta Rays 1:04.47L | 0.70 | 55.24 |
| 30Heat — | Zack Kusch | Streamline Aquat | 0.69 | 55.24 |
| 31Heat — | Patrick MacKill | Atlantis Swimmin | 0.63 | 55.39 |
| 32Heat — | Jack Hathaway | Cavalier Aquatic | 0.73 | 55.52 |
| 33Heat — | Alston Qin | Bolles School Sh | 0.68 | 55.56 |
| 34Heat — | Liam Aleman | Gator Swim Club- 1:04.83L | 0.62 | 55.59 |
| 35Heat — | Max Maycher | New Jersey Race- 1:05.23L | 0.66 | 55.65 |
| 36Heat — | Max Boylan | Excel Aquatics-S 1:05.10L | 0.70 | 55.67 |
| 37Heat — | Jack Gessner | SwimMAC Carolina | 0.63 | 55.68 |
| 38Heat — | Gerhardt Hoover | Commonwealth Swi | 0.65 | 55.69 |
| 39Heat — | Danzhel Gabriel | Patriot Aquatics | 0.59 | 55.71 |
| 40Heat — | Evan Hepburn | YMCA of the Tria 1:04.91L | 0.67 | 55.83 |
| 41Heat — | Spencer Ridge | Nova of Virginia 1:05.28L | 0.66 | 55.86 |
| 42Heat — | Alex Townsend | Marlins Of Ralei | 0.67 | 55.86 |
| 43Heat — | Jordan Willis | SwimMAC Carolina | 0.70 | 55.87 |
| 44Heat — | Cody Ano | Biloxi Elite Swi | 0.68 | 55.89 |
| 45Heat — | Wyatt Porch | Bolles School Sh | 0.63 | 55.97 |
| 46Heat — | Kaden Fatta | York YMCA-MA 1:05.42L | 0.66 | 56.09 |
| 47Heat — | Jake Lloyd | Central Ohio Aqu | 0.62 | 56.09 |
| 48Heat — | Kyle Li | Dragon Swim Team 1:04.96L | 0.62 | 56.11 |
| 49Heat — | Griffin Barlow | Cincinnati Marli 1:05.44L | 0.70 | 56.11 |
| 50Heat — | Jimmy Rogers | Kentucky Aquatic 1:05.46L | 0.70 | 56.11 |
| 51Heat — | Owen Durham | Lakeside Swim Te 1:05.54L | 0.66 | 56.17 |
| 52Heat — | Hayden Lynam | Fort Wayne Swim- 1:05.39L | 0.63 | 56.20 |
| 53Heat — | Matthew Doty | SwimMAC Carolina | 0.59 | 56.20 |
| 54Heat — | Connor Bennett | Mako Swim Team-O 1:05.08L | 0.70 | 56.22 |
| 55Heat — | Matthew Liu | Eagle Swim Team- | 0.69 | 56.23 |
| 56Heat — | Bence Burton | Enfinity Aquatic | 0.70 | 56.25 |
| 57Heat — | Derek Feldman | Central Bucks Sw | 0.66 | 56.26 |
| 58Heat — | Sawyer Lehman | Northridge Area- 1:05.50L | 0.71 | 56.38 |
| 59Heat — | Joe Warnagiris | Winchester Swim- 1:05.99L | 0.63 | 56.38 |
| 60Heat — | Collin Huston | Southeastern Swi 1:04.01L | 0.63 | 56.39 |
| 61Heat — | Nick Marrero | Bolles School Sh 1:04.93L | 0.67 | 56.39 |
| 62Heat — | Tyler Burton | Enfinity Aquatic 1:05.40L | 0.73 | 56.48 |
| 63Heat — | Holden Skidmore | Eagle Swim Team- 1:04.82L | 0.63 | 56.50 |
| 64Heat — | Samuel Martin | Excel Aquatics-S 1:05.23L | 0.67 | 56.51 |
| 65Heat — | Damien Figuet K | Commonwealth Swi 1:04.54L | 0.69 | 56.51 |
| 66Heat — | Gavin Tran | Hershey Aquatic- 1:05.55L | 0.63 | 56.52 |
| 67Heat — | Parker Van Olst | New Wave Swim Te 1:05.54L | 0.68 | 56.52 |
| 68Heat — | Brandon Anand | Scarlet Aquatics 1:04.99L | 0.75 | 56.53 |
| 69Heat — | Lucas Ackermann | Franklin Regiona 1:06.54L | 0.69 | 56.63 |
| 70Heat — | Oliver Hulse | Upper Arlington- 1:05.21L | 0.68 | 56.66 |
| 71Heat — | Tre Cottrell | South Jersey Aqu 1:05.63L | 0.69 | 56.68 |
| 72Heat — | Maddex Ternes | Y-Spartaquatics- | 0.66 | 56.74 |
| 73Heat — | Coleman Kimmel | Memphis Tiger Sw 1:05.41L | 0.66 | 56.77 |
| 74Heat — | Kai Shinkyo | Revolution Aquat | 0.69 | 56.84 |
| 75Heat — | Liam Quigley | Nittany Lion Aqu 1:05.92L | 0.70 | 56.85 |
| 76Heat — | Alex Park | Auburn Aquatics- | 0.66 | 56.86 |
| 77Heat — | Issac Adanin | Club Wolverine-M | 0.75 | 56.87 |
| 78Heat — | Elias Panachyda | North Penn Aquat 1:04.51L | 0.67 | 56.88 |
| 79Heat — | Evan Summers | Nittany Lion Aqu 1:05.31L | 0.69 | 56.89 |
| 80Heat — | Owen Fritts | South Carolina S | 0.60 | 56.94 |
| 81Heat — | Calvin Schaper | Carolina Aquatic 1:04.64L | 0.66 | 56.98 |
| 82Heat — | Sebastian Tith | Upper Main Line- | 0.64 | 56.99 |
| 83Heat — | Connor Lauritze | Club Wolverine-M 1:05.69L | 0.70 | 57.00 |
| 84Heat — | Jihoon Jung | Fishers Area Swi 1:05.41L | 0.58 | 57.05 |
| 85Heat — | Alex Primavera | Bolles School Sh | 0.61 | 57.07 |
| 86Heat — | Gabe Hardy | Mecklenburg Swim 1:05.09L | 0.64 | 57.12 |
| 87Heat — | Matthew Wolfle | Nova of Virginia | 0.68 | 57.21 |
| 88Heat — | Josh Wilder | Team Greenville- | 0.65 | 57.24 |
| 89Heat — | Bobby Regan | Regional YMCA of | 0.63 | 57.25 |
| 90Heat — | Max Little | Greater Pensacol | 0.72 | 57.25 |
| 91Heat — | Vito Cappiello | SwimAtlanta-GA 1:05.57L | 0.66 | 57.33 |
| 92Heat — | Kaleb James | Marlins Of Ralei | 0.64 | 57.34 |
| 93Heat — | Jonathan Vogt | Enfinity Aquatic 1:06.65L | 0.69 | 57.40 |
| 94Heat — | Thor Johannesse | Blazing Barracud 1:07.30L | 0.66 | 57.41 |
| 95Heat — | Cody Miles | Stingrays | 0.73 | 57.45 |
| 96Heat — | Ruihan Zhu | Indiana Swim Clu | 0.67 | 57.54 |
| 97Heat — | Christopher Rec | Tennessee Aquati 1:07.06L | 0.60 | 57.57 |
| 98Heat — | Nate Stebbins | Birmingham 'y' B 1:06.80L | 0.70 | 57.57 |
| 99Heat — | Isaac Holtham | Mecklenburg Swim 1:05.55L | 0.71 | 57.66 |
| 100Heat — | Elijah Holliman | Baylor Swim Club | 0.75 | 57.71 |
| 101Heat — | Gavin Fu | Excel Aquatics-S 1:05.81L | 0.62 | 57.78 |
| 102Heat — | Chen Jd | Nova of Virginia | 0.61 | 57.80 |
| 103Heat — | Asher Allebach | SwimMAC Carolina | 0.69 | 57.84 |
| 104Heat — | Brayden Frankli | Y-Spartaquatics- | 0.69 | 57.86 |
| 105Heat — | Owen Howell | Fox Chapel Kille 1:05.84L | 0.72 | 57.89 |
| 106Heat — | Jack Lynch | Peachtree Aquati 1:05.85L | 0.65 | 57.92 |
| 107Heat — | Trip Cooler | SwimMAC Carolina | 0.63 | 58.16 |
| 108Heat — | Dominic Hilton | Sarasota Sharks- 1:07.31L | 0.71 | 58.27 |
| 109Heat — | Will Kaminsky | SwimAtlanta-GA 1:07.12L | 0.60 | 58.58 |
| 110Heat — | Kian Olsson | Northern Dutches | 0.63 | 58.65 |
| 111Heat — | Logan Poswinski | Fort Wayne Swim- | 0.62 | 58.68 |
| 112Heat — | Jake Jones | Pine Crest Swimm | 0.62 | 58.94 |
| 113Heat — | Ian Supplee | Unattached-SE 1:05.74L | 0.65 | 1:00.06 |
| —Heat — | Fin McLane | Commonwealth Swi | — | |
| —Heat — | Mack Schumann | Nashville Aquati | — | |