| 1Heat — | Koby Bujak-Upto | Unattached-SE 1:49.65L | 0.68 | 1:35.46 |
| 2Heat — | Ethan Ekk | Area Tallahassee | 0.73 | 1:35.61 |
| 3Heat — | Xavier Sohovich | Bolles School Sh | 0.64 | 1:35.85 |
| 4Heat — | Norvin Clontz | Mecklenburg Swim | 0.77 | 1:35.88 |
| 5Heat — | Kenneth Barnicl | Greater Somerset | 0.64 | 1:35.99 |
| 6Heat — | Alex Thiesing | Lakeside Swim Te | 0.69 | 1:36.00 |
| 7Heat — | Luke Sandberg | Dynamo Swim Club | 0.71 | 1:36.04 |
| 8Heat — | Jason Zhao | Mason Manta Rays | 0.64 | 1:36.15 |
| 9Heat — | Liam Carrington | Bolles School Sh | 0.67 | 1:36.27 |
| 10Heat — | Ethan Guo | Greenwood Swimmi | 0.65 | 1:36.43 |
| 11Heat — | Nathan Szobota | Nova of Virginia | 0.69 | 1:36.69 |
| 12Heat — | Will Browne | Cavalier Aquatic | 0.68 | 1:37.05 |
| 13Heat — | Raphael Sibuet | Mercersburg Acad | 0.67 | 1:37.15 |
| 14Heat — | Charlie Kulp | Metro Area Life- | 0.63 | 1:37.49 |
| 15Heat — | Andty Kravchenk | Bolles School Sh 1:53.87L | 0.60 | 1:37.51 |
| 16Heat — | Andrew Malaj | Sarasota Tsunami | 0.62 | 1:37.54 |
| 17Heat — | Sam Huggins | Nittany Lion Aqu | 0.82 | 1:37.58 |
| 18Heat — | Connor Christop | SwimAtlanta | 0.64 | 1:38.11 |
| 19Heat — | Michael Rice | Mecklenburg Swim | 0.59 | 1:38.22 |
| 20Heat — | Jonny Hines | Fishers Area Swi | 0.78 | 1:38.22 |
| 21Heat — | Owen Ekk | Area Tallahassee | 0.61 | 1:38.26 |
| 22Heat — | Hayden Hall | Tennessee Aquati | 0.63 | 1:38.37 |
| 23Heat — | Ethan Zhang | Carmel Swim Club 1:52.94L | 0.69 | 1:38.47 |
| 24Heat — | Matthew Rose | Arlington Aquati | 0.78 | 1:38.51 |
| 25Heat — | Ryan Griffith | Coast Guard Blue 1:53.40L | 0.61 | 1:38.53 |
| 26Heat — | Charlie Zuhoski | Bolles School Sh | 0.71 | 1:38.56 |
| 27Heat — | Carter Hadley | Carmel Swim Club | 0.65 | 1:38.57 |
| 28Heat — | William Allen | Carmel Swim Club 1:52.34L | 0.66 | 1:38.63 |
| 29Heat — | David Melnychuk | Sarasota Sharks- | 0.66 | 1:38.65 |
| 30Heat — | Will Cicco | Utica Shelby Swi 1:54.71L | 0.73 | 1:38.82 |
| 31Heat — | Edward Zhang | Club Wolverine-M | 0.65 | 1:38.83 |
| 32Heat — | Nick Cavic | Commonwealth Swi | 0.67 | 1:38.90 |
| 33Heat — | Oliver Stull | Y-Spartaquatics- 1:54.65L | 0.67 | 1:38.92 |
| 34Heat — | George Dovellos | Bolles School Sh | 0.70 | 1:38.95 |
| 35Heat — | Nicholas Kwan | Bolles School Sh 1:52.60L | 0.70 | 1:39.00 |
| 36Heat — | Tre Cottrell | South Jersey Aqu | 0.71 | 1:39.06 |
| 37Heat — | Wells Cloud | City of Charlest | 0.65 | 1:39.08 |
| 38Heat — | Nick Martin | SwimMAC Carolina 1:55.11L | 0.65 | 1:39.15 |
| 39Heat — | Walter Kueffer | Southwest Stars- 1:54.77L | 0.61 | 1:39.22 |
| 40Heat — | Lewis Zhang | Carmel Swim Club 1:54.05L | 0.72 | 1:39.29 |
| 41Heat — | London Rising | Greater Toledo A 1:52.16L | 0.62 | 1:39.32 |
| 42Heat — | John Owen | Lakeside Swim Te 1:54.04L | 0.69 | 1:39.36 |
| 43Heat — | Marvin Johnson | McCallie / Gps A | 0.62 | 1:39.39 |
| 44Heat — | Walker Lanoue | Planet Swim Aqua | 0.68 | 1:39.44 |
| 45Heat — | Cody Lonsberry | Indian Valley Fa | 0.67 | 1:39.46 |
| 46Heat — | Drew Davis | Marlins Of Ralei 1:55.02L | 0.72 | 1:39.54 |
| 47Heat — | Anthony Pineiro | Eagle Aquatics-F 1:53.16L | 0.62 | 1:39.56 |
| 48Heat — | Thomas Cechini | Mercersburg Acad 1:55.18L | 0.64 | 1:39.63 |
| 49Heat — | Ian Malone | Greater Pensacol | 0.71 | 1:39.66 |
| 50Heat — | Denzo Senekal | Blue Dolfins-FL 1:55.86L | 0.76 | 1:39.67 |
| 51Heat — | Jake Tarara | Zionsville Swim- | 0.71 | 1:39.69 |
| 52Heat — | Tristan Furlow | Shawmut Aquatic- 1:54.42L | 0.70 | 1:39.71 |
| 53Heat — | Kohl Hanes | Naval Academy Aq 1:55.60L | 0.69 | 1:39.71 |
| 54Heat — | George Patterso | Valparaiso Swim- 1:54.27L | 0.65 | 1:39.76 |
| 55Heat — | Andrew Chen | Eagle Swim Team- 1:55.06L | 0.63 | 1:39.82 |
| 56Heat — | Anderson Kopp | Carmel Swim Club 1:55.03L | 0.68 | 1:39.89 |
| 57Heat — | Jack Rutledge | Mecklenburg Swim 1:54.30L | 0.68 | 1:39.95 |
| 58Heat — | Jackson Scheibe | New Wave Swim Te | 0.67 | 1:39.96 |
| 59Heat — | Carter Wright | Bolles School Sh 1:53.92L | 0.66 | 1:39.99 |
| 60Heat — | Oliver Stabach | Indian Valley Fa | 0.65 | 1:40.07 |
| 61Heat — | Cameron York | Area Tallahassee | 0.69 | 1:40.09 |
| 62Heat — | Clay Baumann | Lakeside Swim Te 1:55.00L | 0.65 | 1:40.10 |
| 63Heat — | Colin Jacobs | Unattached-FL 1:55.87L | 0.76 | 1:40.24 |
| 64Heat — | Albert Smelzer | Greensboro Swimm | 0.68 | 1:40.26 |
| 65Heat — | Rawlings Leachm | Woodberry Forest 1:57.30L | 0.65 | 1:40.27 |
| 66Heat — | Santi Alzate | SwimMAC Carolina | 0.73 | 1:40.40 |
| 67Heat — | Gavin Coggan | Zionsville Swim- 1:55.13L | 0.68 | 1:40.41 |
| 68Heat — | Hayden Meyers | Mason Manta Rays | 0.63 | 1:40.42 |
| 69Heat — | Cameron Petitt | Buckeye Swim Clu 1:54.97L | 0.70 | 1:40.49 |
| 70Heat — | Burak Iloglu | Scarlet Aquatics 1:54.09L | 0.72 | 1:40.52 |
| 71Heat — | Will Mulgrew | Shawmut Aquatic- 1:54.47L | 0.74 | 1:40.57 |
| 72Heat — | Hayden Lynam | Fort Wayne Swim- | 0.62 | 1:40.57 |
| 73Heat — | Owen Lin | Greensboro Swimm | 0.61 | 1:40.65 |
| 74Heat — | Owen Waibel | Fanwood-Scotch P | 0.70 | 1:40.67 |
| 75Heat — | Ethan Zhou | Condors Swim Clu | 0.64 | 1:40.74 |
| 76Heat — | Mathias Esteves | Gulliver Swim Cl 1:54.15L | 0.65 | 1:40.79 |
| 77Heat — | Will Charlton | Cavalier Aquatic 1:53.93L | 0.68 | 1:40.80 |
| 78Heat — | Amir Tasker | Chambersburg Cha 1:55.21L | 0.67 | 1:40.87 |
| 79Heat — | Aaron Davidson | New Wave Swim Te 1:54.78L | 0.72 | 1:40.88 |
| 80Heat — | Cannon Martenso | Dynamo Swim Club | 0.68 | 1:41.02 |
| 81Heat — | Samuel Brozek | Sarasota Tsunami 1:54.22L | 0.69 | 1:41.04 |
| 82Heat — | Nazar Zoukovski | Team Pittsburgh- 1:54.58L | 0.64 | 1:41.09 |
| 83Heat — | Jack Eaddy | Revolution Aquat 1:57.52L | 0.74 | 1:41.17 |
| 84Heat — | Mac Clark | Team Pittsburgh- 1:54.96L | 0.80 | 1:41.23 |
| 85Heat — | Cole Nelson | Nova of Virginia 1:56.60L | 0.72 | 1:41.52 |
| 86Heat — | Jackson Debruin | Sarasota Sharks- 1:54.66L | 0.69 | 1:41.54 |
| 87Heat — | Jacob Kim | Scarlet Aquatics 1:55.07L | 0.70 | 1:41.58 |
| 88Heat — | Murray Reed | City of Mobile S 1:56.56L | 0.67 | 1:41.59 |
| 89Heat — | Arjun Velayutha | Mason Manta Rays 1:55.12L | 0.67 | 1:41.62 |
| 90Heat — | Michael McCarth | Jersey Wahoos-MA 1:55.99L | 0.71 | 1:41.62 |
| 91Heat — | Walter Zeman | Laker Swim-FL 1:57.67L | 0.66 | 1:41.63 |
| 92Heat — | Duke Garvin | Crimson Aquatics | 0.71 | 1:41.64 |
| 93Heat — | Brady Campbell | Mason Manta Rays | 0.65 | 1:41.65 |
| 94Heat — | Collier Stanton | Gwinnett Aquatic | 0.67 | 1:41.77 |
| 95Heat — | Lucas Knapp | Bolles School Sh 1:55.25L | 0.70 | 1:41.80 |
| 96Heat — | Luke Waldrep | Baylor Swim Club | 0.62 | 1:41.80 |
| 97Heat — | Emre Sungur | Phoenix Aquatic- 1:53.49L | 0.70 | 1:41.82 |
| 98Heat — | Arnas Monkelis | West Florida Lig 1:54.73L | 0.66 | 1:41.87 |
| 99Heat — | Meyer Hitch | Lakeside Swim Te | 0.71 | 1:41.91 |
| 100Heat — | Andy Pleiman | Northern KY Clip 1:57.06L | 0.72 | 1:42.02 |
| 101Heat — | Andre Brooks | All Star Aquatic | 0.71 | 1:42.03 |
| 102Heat — | Carson Day | SwimMAC Carolina 1:54.14L | 0.71 | 1:42.07 |
| 103Heat — | Luke Hartman | Team Pittsburgh- 1:54.33L | 0.73 | 1:42.17 |
| 104Heat — | Landon Harman | Greater Toledo A 1:57.13L | 0.71 | 1:42.38 |
| 105Heat — | George Groves | Aiken-Augusta Sw | 0.67 | 1:42.49 |
| 106Heat — | Brayden King | Cavalier Aquatic 1:57.56L | 0.70 | 1:42.62 |
| 107Heat — | Carter Rankin | Lakeside Swim Te 1:54.94L | 0.69 | 1:42.67 |
| 108Heat — | Gavin Abelende | Seahawk Aquatics | 0.63 | 1:42.78 |
| 109Heat — | Lincoln Council | Y-Spartaquatics- 1:57.25L | 0.70 | 1:42.78 |
| 110Heat — | Palmer Bice | Naval Academy Aq | 0.63 | 1:43.68 |
| 111Heat — | Boone Wilcox | Lakeside Swim Te 1:55.76L | 0.68 | 1:43.76 |
| 112Heat — | Luke Hamon | Team Greenville- | 0.68 | 1:43.85 |
| 113Heat — | Jack McCrae | SwimMAC Carolina 1:57.31L | 0.74 | 1:43.87 |
| 114Heat — | Luke Parsons | Fishers Area Swi | 0.83 | 1:44.36 |
| 115Heat — | Dillon Brigman | Bolles School Sh 1:55.44L | 0.66 | 1:45.04 |
| 116Heat — | Roberto Sebasti | Southwest Stars- | 0.72 | 1:46.17 |
| —Heat — | Granger Bartee | Mecklenburg Swim | — | |
| —Heat — | John Kroll | SwimMAC Carolina | — | |
| —Heat — | Enzo Desviat Ru | Shawmut Aquatic- | — | |
| —Heat — | Sam Marsteiner | New Wave Swim Te | — | |
| —Heat — | Sam Campbell | Club Wolverine-M | — | NS |