| 1Heat — | Noah Cakir | Team Suffolk | — | 53.62 |
| 2Heat — | Alexei Avakov | Phoenix Swimming | — | 53.81 |
| 3Heat — | Jake Wang | Suburban Seahawk | — | 54.05 |
| 4Heat — | Grayson Nye | Tac Titans | — | 54.07 |
| 5Heat — | Xavier Ruiz | Ridgefield Aquat | — | 54.16 |
| 6Heat — | Austin Carpenter | Central Ohio Aqu | — | 54.21 |
| 7Heat — | Carter Lancaster | Bolles School Sh | — | 54.23 |
| 8Heat — | Luke Vickers | David YMCA Phant | — | 54.24 |
| 9Heat — | Thomas Heilman | CA - Y | — | 54.40 |
| 10Heat — | Jason Zhao | Mason Manta Rays | — | 54.53 |
| 11Heat — | Jordan Willis | SwimMAC Carolina | — | 54.54 |
| 12Heat — | Seneca Oddo | Kentucky Aquatic | — | 54.55 |
| 13Heat — | Caiden Bowers | Eagle Swim Team- | — | 54.58 |
| 14Heat — | Breno Santana | Azura Florida Aq 1:04.46L | — | 54.63 |
| 15Heat — | Max Moore | CA - Y | — | 54.63 |
| 16Heat — | Joseph Gardner | Mount Pleasant A | — | 54.64 |
| 17Heat — | Collin Holgerson | SwimAtlanta-GA 1:05.73L | — | 54.88 |
| 18Heat — | Geoffrey Cradock | Naval Academy Aq 1:06.52L | — | 54.92 |
| 19Heat — | Trent Hollandsworth | H2okie Aquatics- 1:06.16L | — | 54.97 |
| 20Heat — | Wyatt Porch | Bolles School Sh | — | 54.98 |
| 21Heat — | Brandon Malicki | Carmel Swim Club | — | 55.03 |
| 22Heat — | John McNamara | Bolles School Sh | — | 55.07 |
| 23Heat — | Cohen Chiam | Bolles School Sh | — | 55.13 |
| 24Heat — | Javier Colmenares | South Florida Aq | — | 55.24 |
| 25Heat — | Max Nechydyuk | SwimMAC Carolina 1:05.58L | — | 55.25 |
| 26Heat — | Gabe Nunziata | Old Dominion Aqu 1:03.97L | — | 55.25 |
| 27Heat — | Gray Attmore | Nashville Aquati 1:04.94L | — | 55.28 |
| 28Heat — | Eldad Zamir | Bolles School Sh | — | 55.31 |
| 29Heat — | Erik Nadecki | Bgc-N. Westchest | — | 55.33 |
| 30Heat — | Daniel Listor | Peddie Aquatic A | — | 55.36 |
| 31Heat — | Jayden Repak | Berkeley Aquatic | — | 55.43 |
| 32Heat — | Eli Greinke | Kentucky Aquatic 1:05.46L | — | 55.47 |
| 33Heat — | Ian Call | Memphis Thunder- 1:02.22L | — | 55.51 |
| 34Heat — | Pablo Silva | Nova of Virginia 1:04.83L | — | 55.53 |
| 35Heat — | Lucas Young | Hurricane Aquati 1:04.97L | — | 55.57 |
| 36Heat — | Max Buchen | Three Village Sw 1:05.40L | — | 55.59 |
| 37Heat — | Noah Saylor | SwimAtlanta-GA 1:05.00L | — | 55.62 |
| 38Heat — | Luke Newcomb | Club Wolverine-M | — | 55.63 |
| 39Heat — | Salvatore Lanzafame | Azura Florida Aq 1:05.52L | — | 55.76 |
| 40Heat — | Jay Schlosser | Nashville Aquati | — | 55.78 |
| 41Heat — | Reed Lucero | Unattached-OH/U | — | 55.85 |
| 42Heat — | Alex Gallagher | Hudson Explorer- 1:05.06L | — | 55.87 |
| 43Heat — | Alex Cucu | SwimUnited | — | 55.96 |
| 44Heat — | Cole Stevenson | Irish Aquatics-I | — | 55.98 |
| 45Heat — | Christian Losh | SwimMAC Carolina 1:03.84L | — | 56.00 |
| 46Heat — | Jack Ussery | Hilton Head Aqua 1:05.03L | — | 56.01 |
| 47Heat — | Ian Fournier | Kennebec Valley- | — | 56.02 |
| 47Heat — | Louis Joos | Bolles School Sh 1:04.86L | — | 56.02 |
| 49Heat — | Doyee Kim | Unattached | — | 56.04 |
| 50Heat — | Gerhardt Hoover | Commonwealth Swi | — | 56.10 |
| 51Heat — | George Groves | Gator Swim Club- 1:06.03L | — | 56.17 |
| 52Heat — | Wilson York | Lakeside Swim Te 1:04.09L | — | 56.20 |
| 53Heat — | Owen Johanssen | Unattached-OH/U 1:05.99L | — | 56.21 |
| 54Heat — | Alex Primavera | Bolles School Sh | — | 56.30 |
| 55Heat — | Tiernan Moore | Kentucky Aquatic 1:06.38L | — | 56.38 |
| 56Heat — | Jonah Lee | Valparaiso Swim- 1:04.81L | — | 56.40 |
| 57Heat — | Luke Mychalowych | Motor City Aquat | — | 56.41 |
| 57Heat — | Jack Gessner | SwimMAC Carolina 1:06.27L | — | 56.41 |
| 59Heat — | Nate Hohm | Aquatic Team Of- | — | 56.42 |
| 60Heat — | Ryan Frank | Mount Lebanon Aq 1:06.11L | — | 56.43 |
| 61Heat — | Michael Gorey | Carmel Swim Club 1:06.00L | — | 56.48 |
| 62Heat — | Greg Kisshauer | Mecklenburg Swim 1:06.16L | — | 56.49 |
| 63Heat — | Liam Aleman | Gator Swim Club- 1:05.77L | — | 56.51 |
| 64Heat — | Jude Banks | Lake Erie Silver 1:05.66L | — | 56.55 |
| 65Heat — | Seth Braun | Northern Tribs S | — | 56.57 |
| 66Heat — | Calvin Schaper | Carolina Aquatic 1:06.31L | — | 56.63 |
| 67Heat — | Holden Skidmore | Eagle Swim Team- 1:06.14L | — | 56.64 |
| 68Heat — | Cutter Shipman | Martin County Sw 1:06.59L | — | 56.71 |
| 69Heat — | Spencer Ridge | Nova of Virginia 1:06.34L | — | 56.76 |
| 69Heat — | Cameron Frazier | Westerville Aqua 1:06.11L | — | 56.76 |
| 71Heat — | Gavin McCarter | SwimMAC Carolina | — | 56.77 |
| 72Heat — | Noel Tumbasz | Central Ohio Aqu | — | 56.78 |
| 73Heat — | Zach Drotar | Cato-Meridian | — | 56.83 |
| 74Heat — | Daniel Seong | Phoenix Swimming 1:05.00L | — | 56.91 |
| 75Heat — | Collin Huston | Southeastern Swi 1:05.61L | — | 56.93 |
| 76Heat — | Robert Alvarez | Metro Aquatic Cl 1:06.04L | — | 56.99 |
| 77Heat — | Benjamin Berry | McCallie / Gps A | — | 57.07 |
| 78Heat — | Luke Zardavets | Planet Swim Aqua | — | 57.17 |
| 79Heat — | Griffin Barlow | Cincinnati Marli 1:05.30L | — | 57.23 |
| 80Heat — | Carter Miller | Ensworth Aquatic | — | 57.32 |
| 81Heat — | Owen Vonweihe | Marlins Of Ralei 1:05.04L | — | 57.33 |
| 82Heat — | Carrick Shea | Crimson Aquatics 1:06.34L | — | 57.34 |
| 83Heat — | Elias Corn | Sarasota Tsunami 1:06.37L | — | 57.37 |
| 84Heat — | Danzhel Tuliao | Patriot Aquatics 1:05.11L | — | 57.43 |
| 85Heat — | Dante Buonadonna | Jersey Wahoos-MA 1:06.34L | — | 57.45 |
| 86Heat — | Harper Johnson | Dynamo Swim Club 1:05.94L | — | 57.50 |
| 87Heat — | Matthew Doty | SwimMAC Carolina 1:06.15L | — | 57.52 |
| 88Heat — | Emilio Aparicio | Raleigh Swimming | — | 57.58 |
| 89Heat — | Anthony Koh | Penn Charter Aqu 1:06.45L | — | 57.59 |
| 90Heat — | Kyle Li | Unattached-PV 1:06.25L | — | 57.60 |
| 91Heat — | Alexander Townsend | Marlins Of Ralei | — | 57.62 |
| 92Heat — | Owen Durham | Lakeside Swim Te 1:06.18L | — | 57.65 |
| 92Heat — | Christian Dantey | Fox Chapel Kille 1:05.77L | — | 57.65 |
| 94Heat — | Carter Robertson | Team Pittsburgh- 1:05.39L | — | 57.68 |
| 95Heat — | Tre Cottrell | South Jersey Aqu 1:06.33L | — | 57.82 |
| 95Heat — | Brett Bailey | Southern Tide Sw | — | 57.82 |
| 97Heat — | Coleman Kimmel | Memphis Tiger Sw 1:05.22L | — | 57.86 |
| 98Heat — | Samuel Martin | Excel Aquatics-S 1:06.20L | — | 57.96 |
| 99Heat — | Andrew Delzer | Oly Swimming-MI 1:05.13L | — | 57.99 |
| 100Heat — | Thomas Fenton | Lynchburg YMCA S 1:06.23L | — | 58.00 |
| 101Heat — | Asher Allebach | SwimMAC Carolina 1:06.52L | — | 58.02 |
| 102Heat — | Isaac Holtham | Life Time North 1:06.23L | — | 58.13 |
| 103Heat — | Owen Howell | Fox Chapel Kille 1:06.40L | — | 58.14 |
| 104Heat — | Max Mahler | Enfinity Aquatic 1:06.31L | — | 58.20 |
| 105Heat — | Edward Zhang | Club Wolverine-M 1:06.08L | — | 58.24 |
| 106Heat — | Ryder Hicks | Mason Manta Rays | — | 58.30 |
| 107Heat — | Elliot Elmore | Stingrays | — | 58.34 |
| 108Heat — | Aleksandr Ries | Franklin Regiona | — | 58.41 |
| 108Heat — | Eric Rein | Academy Aquatic- 1:06.51L | — | 58.41 |
| 110Heat — | Gavin Tran | Hershey Aquatic- 1:06.88L | — | 58.57 |
| 111Heat — | Walker Bailey | Cincinnati Marli 1:06.16L | — | 58.88 |
| 112Heat — | Ian Disosway | Fast Falcons-FL 1:07.44L | — | 59.42 |
| 113Heat — | George Gonzalez | Unattached | — | 59.49 |
| 114Heat — | Noah Roettger | Tennessee Aquati 1:06.55L | — | 59.96 |
| —Heat — | Peter Peyser | Star Swimming | — | DQ |
| —Heat — | Adam McCurdy | Unattached-OH/U | — | DQ |
| —Heat — | Eric Lee | Scarlet Aquatics | — | |